@TOME V2.3
(Mar 2018)
Ref.
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Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : spr1888: (2018-01-22 )
MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAAQILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVVKREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTLVCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQTASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLFWLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYDIERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELESLISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTLKIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSANLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQIDIRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQLGSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDELGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQDGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQTSAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL
Atome Classification :
(24 SA)
.........10........20........30........40........50........60........70........80........90........100.......110.......120..-......130.......140.......150.......160.......170.......180....-...190........----200..-.....---210.-..-------...-----..220.......230.......240.......250.......260.......270.......280.......290.......300.......310.......320.......330.......340.......350.......360.......-370.......380.......390.......400.......410.......420.......430.......440.......450.......460.......470.......480.......490.......500.......510.......520.......530.......540.......550.......560.......570.......580.......590.......600.......610.......620.......630.......640.......650.......660.......670.......680.......690.......700.......710.......720.......730.......740.......750.......760.......770.......780.......790.......800.......810.......820.......830.......840..
(Atome)
(Ident)
(Tito)
(Num)
(QMean)
(Ligand)
(Uniprot)
M
A
I
E
KLS
P
G
M
Q
Q
Y
V
D
I
K
K
QYP
D
A
F
L
L
FRM
G
D
F
Y
E
L
F
Y
E
DAVN
A
A
Q
I
L
E
I
S
L
TSRNKNADNPIPMAGVPY
H
S
A
Q
Q
Y
I
D
V
L
I
EQG
Y
K
V
A
I
A
E
Q
ME
D
P
K
Q
A
V
G
V
V
K
R
E
V
V
Q
VITPGTVVDSSK-PDS-QN
N
F
L
V
S
IDREGNQF
G
L
A
Y
M
D
LVTG
D
F
Y
V
T
G
L
L
D
F
T
L
V
C
G
E
IRNL
K
A
R
E
V
V
LGYDL
S
E
E
E
E
Q
I
-
L
S
R
Q
-
M
N
L
V
L
S
YEK----ESFED-
L
H
L
L
D
---
L
R
-
L
-
A
T
-------
V
E
Q
-----
T
A
S
S
K
L
L
Q
Y
V
H
R
T
Q
M
R
E
L
N
HL
K
P
V
I
R
Y
E
I
K
D
F
L
QMDYATKA
S
L
D
L
V
E
N
A
RSGKKQG
S
L
F
W
L
LDE
T
K
T
A
M
G
M
R
L
L
R
S
W
IHRPLI
D
K
E
R
I
V
Q
R
Q
E
V
V
Q
V
F
L
D
H
F
F
E
RSD
L
T
D
S
L
K
G
V
Y
D
I
E
R
L
A
S
R
VSFGKT
N
P
K
D
L
L
Q
L
A
T
T
L
SS
V
P
R
I
R
A
I
L
-EGMEQ
P
T
L
A
Y
L
I
A
Q
L
DA
I
P
E
L
E
S
L
I
S
A
AIAPEA
P
H
V
ITDG
G
I
I
RT
G
F
D
E
T
L
D
K
Y
R
C
V
L
REGT
S
W
I
A
E
I
E
A
K
ERENSG
I
S
T
L
K
I
D
YNKKD
G
Y
Y
F
HVTNSQLGNV
P
A
H
F
F
R
K
A
TLKNSERF
G
T
E
E
L
A
R
IE
G
D
M
L
E
A
R
E
KSANL
E
Y
E
I
F
M
R
I
R
E
E
V
G
K
Y
I
Q
R
L
Q
A
L
A
Q
G
I
A
T
V
D
V
L
Q
S
L
A
V
V
A
E
T
Q
H
L
IRPEFGDDS
Q
I
D
I
R
KG
R
H
A
V
V
E
K
V
MGAQTYIPN
T
I
Q
MAED
T
S
I
Q
L
V
TGPNMS
G
K
S
T
Y
M
R
Q
L
A
M
T
A
V
M
A
Q
L
GSYVPAESAHL
P
I
F
D
A
I
F
T
RIGAADDLVSGQ
S
T
F
M
V
E
M
MEANNAISHATKN
S
L
I
L
F
DELGRGTATY
D
G
M
A
L
A
Q
S
I
I
E
Y
I
H
E
HIGA
K
T
L
F
A
T
H
Y
H
E
L
T
S
L
E
S
S
L
Q
H
L
V
N
V
H
V
A
T
LEQDG
Q
V
T
F
L
H
KIEPGPADKSYG
I
H
V
A
K
IAGL
P
A
D
L
L
A
R
A
D
K
I
L
T
Q
L
E
NQGTESPPPM
R
Q
T
S
A
V
T
E
Q
I
S
L
F
D
RAEEH
P
I
L
A
E
L
A
K
LDVYNM
T
P
M
Q
V
M
N
V
L
V
E
L
K
QKL
13
PsiBlast_PDB
88.71
39%
-47
- C3 -
-
1EWQ
3
MUTS_THEAQ
A:
[13-752]
-----
L
P
P
L
L
Q
Q
Y
V
E
L
R
D
Q
Y
P
D
Y
L
L
L
F
Q
V
G
D
F
Y
E
C
F
G
E
D
A
E
R
L
A
R
A
L
G
L
V
L
T
H
K
T
S
K
-
D
F
T
T
P
M
A
G
I
P
L
R
A
F
E
A
Y
A
E
R
L
L
K
M
G
F
R
L
A
V
A
D
Q
V
E
P
A
E
E
A
E
G
L
V
R
R
E
V
T
Q
L
L
T
P
G
T
L
L
Q
E
S
L
-
L
P
R
-
E
A
N
Y
L
A
A
I
A
-
T
G
D
G
W
G
L
A
F
L
D
V
S
T
G
E
F
K
G
T
V
L
K
S
K
S
A
L
Y
D
E
L
F
R
H
R
P
A
E
V
L
L
A
P
E
L
L
E
N
G
A
F
L
-
D
E
F
R
-
K
R
F
P
V
M
L
S
E
----
A
P
F
E
P
-
E
G
E
G
P
---
L
A
-
L
------------
R
-----
R
A
R
G
A
L
L
A
Y
A
Q
R
T
Q
G
G
A
L
S
-
L
Q
P
F
R
F
Y
D
P
G
A
F
M
R
L
P
E
A
T
L
R
A
L
E
V
F
E
P
L
R
G
---
Q
D
T
L
F
S
V
L
D
E
T
R
T
A
P
G
R
R
L
L
Q
S
W
L
R
H
P
L
L
D
R
G
P
L
E
A
R
L
D
R
V
E
G
F
V
R
E
G
A
L
R
E
G
V
R
R
L
L
Y
R
L
A
D
L
E
R
L
A
T
R
L
E
L
G
R
A
S
P
K
D
L
G
A
L
R
R
S
L
Q
I
L
P
E
L
R
A
L
L
G
E
E
V
G
L
P
D
L
S
P
L
K
E
E
L
E
A
----------
A
L
V
E
D
P
P
L
K
V
S
E
G
G
L
I
R
E
G
Y
D
P
D
L
D
A
L
R
A
A
H
R
E
G
V
A
Y
F
L
E
L
E
E
R
E
R
E
R
T
G
I
P
T
L
K
V
G
Y
N
A
V
F
G
Y
Y
L
E
V
T
R
P
Y
Y
E
R
V
P
K
E
Y
R
P
V
Q
T
L
K
D
R
Q
R
Y
T
L
P
E
M
K
E
K
E
R
E
V
Y
R
L
E
A
L
I
R
R
R
E
E
E
V
F
L
E
V
R
E
R
A
K
R
Q
A
E
A
L
R
E
A
A
R
I
L
A
E
L
D
V
Y
A
A
L
A
E
V
A
V
R
Y
G
Y
V
R
P
R
F
G
D
--
R
L
Q
I
R
A
G
R
H
P
V
V
E
R
---
R
T
E
F
V
P
N
D
L
E
M
A
H
E
--
L
V
L
I
T
G
P
N
M
A
G
K
S
T
F
L
R
Q
T
A
L
I
A
L
L
A
Q
V
G
S
F
V
P
A
E
E
A
H
L
P
L
F
D
G
I
Y
T
R
I
G
A
------
G
K
S
T
F
M
V
E
M
E
E
V
A
L
I
L
K
E
A
T
E
N
S
L
V
L
L
D
E
V
G
R
G
T
S
S
L
D
G
V
A
I
A
T
A
V
A
E
A
L
H
E
R
R
-
A
Y
T
L
F
A
T
H
Y
F
E
L
T
A
L
--
G
L
P
R
L
K
N
L
H
V
A
A
R
E
E
A
G
G
L
V
F
Y
H
Q
V
L
P
G
P
A
S
K
S
Y
G
V
E
V
A
A
M
A
G
L
P
K
E
V
V
----------------------------------------------------------------------
35
HHSearch
88.35
38%
-21
- C3 -
-
1WB9
3
MUTS_ECOLI
A:
[8-798]
---
D
A
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
T
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
-
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
D
LVG
F
G
V
E
N
A
P
-------
R
G
L
-----
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
-
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
14
PsiBlast_PDB
88.00
39%
-50
- C3 -
-
1FW6
3
MUTS_THEAQ
A:
[13-752]
-----
L
P
P
L
L
Q
Q
Y
V
E
L
R
D
Q
Y
P
D
Y
L
L
L
F
Q
V
G
D
F
Y
E
C
F
G
E
D
A
E
R
L
A
R
A
L
G
L
V
L
T
H
K
T
S
K
-
D
F
T
T
P
M
A
G
I
P
L
R
A
F
E
A
Y
A
E
R
L
L
K
M
G
F
R
L
A
V
A
D
Q
V
E
P
A
E
E
A
E
G
L
V
R
R
E
V
T
Q
L
L
T
P
G
T
L
L
Q
E
S
L
-
L
P
R
-
E
A
N
Y
L
A
A
I
A
-
T
G
D
G
W
G
L
A
F
L
D
V
S
T
G
E
F
K
G
T
V
L
K
S
K
S
A
L
Y
D
E
L
F
R
H
R
P
A
E
V
L
L
A
P
E
L
L
E
N
G
A
F
L
-
D
E
F
R
-
K
R
F
P
V
M
L
S
E
----
A
P
F
E
P
-
E
G
E
G
P
---
L
A
-
L
------------
R
-----
R
A
R
G
A
L
L
A
Y
A
Q
R
T
Q
G
G
A
L
S
-
L
Q
P
F
R
F
Y
D
P
G
A
F
M
R
L
P
E
A
T
L
R
A
L
E
V
F
E
P
L
R
G
---
Q
D
T
L
F
S
V
L
D
E
T
R
T
A
P
G
R
R
L
L
Q
S
W
L
R
H
P
L
L
D
R
G
P
L
E
A
R
L
D
R
V
E
G
F
V
R
E
G
A
L
R
E
G
V
R
R
L
L
Y
R
L
A
D
L
E
R
L
A
T
R
L
E
L
G
R
A
S
P
K
D
L
G
A
L
R
R
S
L
Q
I
L
P
E
L
R
A
L
L
G
E
E
V
G
L
P
D
L
S
P
L
K
E
E
L
E
A
----------
A
L
V
E
D
P
P
L
K
V
S
E
G
G
L
I
R
E
G
Y
D
P
D
L
D
A
L
R
A
A
H
R
E
G
V
A
Y
F
L
E
L
E
E
R
E
R
E
R
T
G
I
P
T
L
K
V
G
Y
N
A
V
F
G
Y
Y
L
E
V
T
R
P
Y
Y
E
R
V
P
K
E
Y
R
P
V
Q
T
L
K
D
R
Q
R
Y
T
L
P
E
M
K
E
K
E
R
E
V
Y
R
L
E
A
L
I
R
R
R
E
E
E
V
F
L
E
V
R
E
R
A
K
R
Q
A
E
A
L
R
E
A
A
R
I
L
A
E
L
D
V
Y
A
A
L
A
E
V
A
V
R
Y
G
Y
V
R
P
R
F
G
D
--
R
L
Q
I
R
A
G
R
H
P
V
V
E
R
---
R
T
E
F
V
P
N
D
L
E
M
A
H
E
--
L
V
L
I
T
G
P
N
M
A
G
K
S
T
F
L
R
Q
T
A
L
I
A
L
L
A
Q
V
G
S
F
V
P
A
E
E
A
H
L
P
L
F
D
G
I
Y
T
R
I
G
A
------
G
K
S
T
F
M
V
E
M
E
E
V
A
L
I
L
K
E
A
T
E
N
S
L
V
L
L
D
E
V
G
R
G
T
S
S
L
D
G
V
A
I
A
T
A
V
A
E
A
L
H
E
R
R
-
A
Y
T
L
F
A
T
H
Y
F
E
L
T
A
L
--
G
L
P
R
L
K
N
L
H
V
A
A
R
E
E
A
G
G
L
V
F
Y
H
Q
V
L
P
G
P
A
S
K
S
Y
G
V
E
V
A
A
M
A
G
L
P
K
E
V
V
----------------------------------------------------------------------
9
PsiBlast_PDB
87.25
37%
-20
- C3 -
-
1WB9
3
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
T
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
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K
M
G
E
F
A
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L
R
D
L
L
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R
A
I
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D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
12
PsiBlast_PDB
87.21
36%
-18
- C3 -
-
1E3M
3
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
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R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
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L
R
D
L
L
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R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
1
PsiBlast_PDB
87.19
37%
-23
- C3 -
-
1OH5
3
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
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N
T
L
A
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V
L
D
C
T
V
T
P
M
G
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M
L
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R
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L
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M
P
V
R
D
T
R
V
L
L
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R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
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L
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R
I
L
A
R
L
A
L
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R
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A
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R
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A
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L
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L
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A
Q
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A
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V
Q
A
L
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M
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A
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L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
8
PsiBlast_PDB
87.14
37%
-19
- C3 -
-
3K0S
3
MUTS_ECOLI
A:
[10-797]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
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T
V
D
S
A
P
V
Q
A
L
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M
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A
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L
R
D
L
L
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A
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D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
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R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
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K
G
K
A
L
A
L
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K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
------------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
N
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
10
PsiBlast_PDB
86.56
37%
-20
- C3 -
-
1WBB
3
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
A
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
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T
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P
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M
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R
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Q
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S
I
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M
D
A
A
T
R
R
N
L
E
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T
Q
N
L
-
A
G
G
A
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N
T
L
A
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V
L
D
C
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P
M
G
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M
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M
P
V
R
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V
L
L
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Q
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G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
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L
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R
I
L
A
R
L
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A
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L
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A
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V
Q
A
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K
M
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A
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R
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L
L
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A
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D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
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R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
4
PsiBlast_PDB
86.41
37%
-18
- C3 -
-
1OH8
3
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
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T
T
L
P
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R
S
I
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M
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R
E
Q
D
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M
D
A
A
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R
R
N
L
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N
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-
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G
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A
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A
L
Q
D
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---
T
A
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L
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L
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L
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A
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V
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A
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A
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P
V
L
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G
V
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A
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Y
N
E
E
L
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A
L
A
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A
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L
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R
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L
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A
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L
L
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A
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A
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L
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L
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V
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-
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R
M
L
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R
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A
L
I
A
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A
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Y
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P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
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Y
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L
V
L
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K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
7
PsiBlast_PDB
86.28
37%
-19
- C3 -
-
1WBD
3
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
Q
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
5
PsiBlast_PDB
86.15
37%
-14
- C3 -
-
2WTU
1
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
A
A
D
--------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
3
PsiBlast_PDB
86.00
37%
-18
- C3 -
-
1OH7
3
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
2
PsiBlast_PDB
85.91
37%
-19
- C3 -
-
1OH6
3
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
30
PsiBlast_CBE
85.90
37%
-16
- C3 -
-
1WB9
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
T
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
6
PsiBlast_PDB
84.48
37%
-13
- C3 -
-
3ZLJ
2
MUTS_ECOLI
A:
[11-798]
------
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
------------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
---------
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
23
PsiBlast_CBE
84.36
37%
-15
- C3 -
-
1W7A
4
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
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Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
A
A
D
D
--
S
G
R
S
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
31
PsiBlast_CBE
84.33
37%
-17
- C3 -
-
1WBB
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
A
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
28
PsiBlast_CBE
84.26
37%
-14
* C3 *
-
1WBD
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
Q
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
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A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
25
PsiBlast_CBE
84.04
37%
-15
- C3 -
-
1OH7
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
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A
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V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
29
PsiBlast_CBE
83.87
37%
-13
- C3 -
-
3K0S
2
MUTS_ECOLI
B:
[17-797]
-------------
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
-----------
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
-
E
T
V
D
S
A
P
V
Q
A
L
R
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M
G
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F
A
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L
R
D
L
L
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R
A
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I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
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W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
---------
S
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
N
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
26
PsiBlast_CBE
83.76
37%
-16
- C3 -
-
1OH6
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
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R
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L
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A
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A
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L
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A
I
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D
T
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P
V
L
V
R
D
G
G
V
I
A
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G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
24
PsiBlast_CBE
83.54
37%
-16
- C3 -
-
1OH8
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
A
P
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
-
A
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
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I
L
A
R
L
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L
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P
V
L
V
R
D
G
G
V
I
A
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G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
27
PsiBlast_CBE
83.20
37%
-17
- C3 -
-
1OH5
2
MUTS_ECOLI
B:
[14-798]
---------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
-
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
M
S
L
I
E
-
G
R
R
G
-
L
R
R
R
P
L
W
E
F
----
E
I
D
T
A
-
R
Q
Q
L
N
---
L
Q
-
F
-
G
T
RDLVGFG
V
E
N
AP
R
G
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
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T
Q
N
L
-
A
G
G
A
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N
T
L
A
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V
L
D
C
T
V
T
P
M
G
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M
L
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P
V
R
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R
V
L
L
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Q
Q
T
I
G
A
L
Q
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---
T
A
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L
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L
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V
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A
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K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
N
-
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
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T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
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I
G
R
G
T
S
T
Y
D
G
L
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L
A
W
A
C
A
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N
L
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N
K
I
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A
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L
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A
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F
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L
P
E
K
M
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V
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V
H
L
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A
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A
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A
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V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------
21
PsiBlast_CBE
81.36
37%
-9
- C3 -
-
3ZLJ
2
MUTS_ECOLI
B:
[26-798]
---------------------
E
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L
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--------------------
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---
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----------------------------------------------------------