@TOME V2.3
(Mar 2018)
Ref.
-
-
Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : I3TYE2: (2017-12-14 )
MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESAELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNCMFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGAGSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDTVTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNKMKEE
Atome Classification :
(45 SA)
.........10........-20.........30........40-.--.--....--...50........60.........70........80........90........100.......110........120.......130.......140.......150.......160.......170........180.......190.......200.......210.......220.......230.......240.......250.......260.......270.......280
(Atome)
(Ident)
(Tito)
(Num)
(QMean)
(Ligand)
(Uniprot)
MTEFK
T
E
K
E
K
M
I
A
G
E
L
Y
FA-N-
D
P
E
L
V
A
D
R
M
F
A
R
S
Q
S
Q
I
I
N
Q
--
A
--
E
--
S
A
E
L
--
R
S
Q
L
L
K
E
T
FGRTG
K
-
K
I
Y
M
E
P
V
I
N
F
DY
G
Y
N
I
F
VGE
N
F
Y
A
N
F
N
C
T
F
L
D
V
S
T
I
EIGDN
C
M
FAPN
V
Q
L
Y
-TATHPLHPVKRNSGLEYA
K
P
IKIGN
N
V
W
LGG
G
V
I
VTPGVTLGD
N
V
V
VGAGS
V
V
TKSFPD-
N
V
V
IAG
N
P
A
R
I
I
K
T
V
E
E
E
L
T
E
E
S
L
E
T
L
R
H
K
I
D
M
I
D
R
E
L
V
A
L
L
E
K
R
M
D
T
V
T
K
I
G
Q
V
KKEENQ
A
V
Y
D
A
K
R
E
Q
Q
V
L
D
K
V
V
S
L
L
KNKGYKE
T
I
T
D
T
Y
V
D
L
M
K
H
S
R
E
Y
Q
N
K
MKEE
140
HHSearch
86.64
59%
-28
- C2 -
-
1OCX
-
MAA_ECOLI
A:
[1-182]
----
S
T
E
K
E
K
M
I
A
G
E
L
Y
R
S
-
A
-
D
E
T
L
S
R
D
R
L
R
A
R
Q
L
I
H
R
Y
N
H
--
S
LA
E
--
E
H
T
L
--
R
Q
Q
I
L
A
D
L
F
G
Q
V
-
T
-
E
A
Y
I
E
P
T
F
R
C
D
Y
G
Y
N
I
F
L
G
N
N
F
F
A
N
F
D
C
V
M
L
D
V
C
P
I
R
I
G
D
N
C
M
L
A
P
G
V
H
I
Y
-
T
A
T
H
P
I
D
P
V
A
R
N
S
G
A
E
L
G
K
P
V
T
I
G
N
N
V
W
I
G
G
R
A
V
I
N
P
G
V
T
I
G
D
N
V
V
V
A
S
G
A
V
V
T
K
D
V
P
D
-
N
V
V
V
G
G
N
P
A
R
I
I
K
K
L
-----------------------------------------------------------------------------------------------
3
PsiBlast_PDB
86.49
59%
-30
- C2 -
-
1OCX
-
MAA_ECOLI
A:
[1-180]
----
S
T
E
K
E
K
M
I
A
G
E
L
Y
R
S
-
A
-
D
E
T
L
S
R
D
R
L
R
A
R
Q
L
I
H
R
Y
N
H
SL
A
--
E
--
E
H
T
L
--
R
Q
Q
I
L
A
D
L
F
G
Q
V
-
T
-
E
A
Y
I
E
P
T
F
R
C
D
Y
G
Y
N
I
F
L
G
N
N
F
F
A
N
F
D
C
V
M
L
D
V
C
P
I
R
I
G
D
N
C
M
L
A
P
G
V
H
I
Y
-
T
A
T
H
P
I
D
P
V
A
R
N
S
G
A
E
L
G
K
P
V
T
I
G
N
N
V
W
I
G
G
R
A
V
I
N
P
G
V
T
I
G
D
N
V
V
V
A
S
G
A
V
V
T
K
D
V
P
D
-
N
V
V
V
G
G
N
P
A
R
I
I
K
-------------------------------------------------------------------------------------------------
136
HHSearch
83.78
57%
-20
- C2 -
-
3SRT
6
?
B:
[2-187]
--
N
A
M
T
E
K
E
K
M
L
S
G
K
G
Y
Y
A
-
N
-
D
E
L
L
V
K
E
R
E
Y
C
K
K
L
T
R
L
F
N
N
--
T
LE
D
--
E
Y
E
K
--
R
E
D
I
L
R
Q
L
F
G
S
V
G
K
-
Q
I
N
V
E
Q
N
I
R
C
D
Y
G
Y
N
I
H
V
G
E
N
F
F
A
N
Y
D
C
I
F
L
D
V
C
K
I
E
I
G
D
N
V
M
L
A
P
N
V
Q
I
Y
-
T
A
Y
H
P
I
D
A
Q
L
R
N
S
G
I
E
Y
G
S
P
V
K
I
G
D
N
V
W
I
G
G
G
V
I
I
T
P
G
I
T
I
G
D
N
V
V
I
G
A
G
S
V
V
T
K
D
I
P
P
-
N
T
V
A
V
G
N
P
C
R
V
I
K
K
I
E
----------------------------------------------------------------------------------------------
137
HHSearch
82.80
57%
-22
- C2 -
-
3SRT
10
?
A:
[3-187]
---
A
M
T
E
K
E
K
M
L
S
G
K
G
Y
Y
A
-
N
-
D
E
L
L
V
K
E
R
E
Y
C
K
K
L
T
R
L
F
N
N
--
T
LE
D
--
E
Y
E
K
--
R
E
D
I
L
R
Q
L
F
G
S
V
G
K
-
Q
I
N
V
E
Q
N
I
R
C
D
Y
G
Y
N
I
H
V
G
E
N
F
F
A
N
Y
D
C
I
F
L
D
V
C
K
I
E
I
G
D
N
V
M
L
A
P
N
V
Q
I
Y
-
T
A
Y
H
P
I
D
A
Q
L
R
N
S
G
I
E
Y
G
S
P
V
K
I
G
D
N
V
W
I
G
G
G
V
I
I
T
P
G
I
T
I
G
D
N
V
V
I
G
A
G
S
V
V
T
K
D
I
P
P
-
N
T
V
A
V
G
N
P
C
R
V
I
K
K
I
E
----------------------------------------------------------------------------------------------
5
PsiBlast_PDB
81.02
56%
-18
- C2 -
-
4ISX
2
?
A:
[5-188]
-----
T
E
K
E
K
M
L
S
G
K
G
Y
Y
A
-
N
-
D
E
L
L
V
K
E
R
E
Y
C
K
K
L
T
R
L
F
N
N
--
T
LE
D
--
E
Y
E
K
--
R
E
D
I
L
R
Q
L
F
G
S
V
G
K
-
Q
I
N
V
E
Q
N
I
R
C
D
Y
G
Y
N
I
H
V
G
E
N
F
F
A
N
Y
D
C
I
F
L
D
V
C
K
I
E
I
G
D
N
V
M
L
A
P
N
V
Q
I
Y
-
T
A
Y
H
P
I
D
A
Q
L
R
N
S
G
I
E
Y
G
S
P
V
K
I
G
D
N
V
W
I
G
G
G
V
I
I
T
P
G
I
T
I
G
D
N
V
V
I
G
A
G
S
V
V
T
K
D
I
P
P
-
N
T
V
A
V
G
N
P
C
R
V
I
K
K
I
E
E
---------------------------------------------------------------------------------------------
4
PsiBlast_PDB
80.96
56%
-21
- C2 -
-
3SRT
10
?
A:
[5-188]
-----
T
E
K
E
K
M
L
S
G
K
G
Y
Y
A
-
N
-
D
E
L
L
V
K
E
R
E
Y
C
K
K
L
T
R
L
F
N
N
--
T
LE
D
--
E
Y
E
K
--
R
E
D
I
L
R
Q
L
F
G
S
V
G
K
-
Q
I
N
V
E
Q
N
I
R
C
D
Y
G
Y
N
I
H
V
G
E
N
F
F
A
N
Y
D
C
I
F
L
D
V
C
K
I
E
I
G
D
N
V
M
L
A
P
N
V
Q
I
Y
-
T
A
Y
H
P
I
D
A
Q
L
R
N
S
G
I
E
Y
G
S
P
V
K
I
G
D
N
V
W
I
G
G
G
V
I
I
T
P
G
I
T
I
G
D
N
V
V
I
G
A
G
S
V
V
T
K
D
I
P
P
-
N
T
V
A
V
G
N
P
C
R
V
I
K
K
I
E
E
---------------------------------------------------------------------------------------------
2
PsiBlast_PDB
79.31
56%
-2
- C2 -
-
3IGJ
6
?
A:
[5-190]
----
K
T
E
K
D
K
M
L
A
G
E
M
Y
I
A
-
D
-
D
E
E
L
V
A
D
R
V
E
A
K
R
L
T
R
L
Y
N
E
--
A
V
E
T
--
G
D
E
R
--
R
F
T
L
L
N
Q
L
L
G
S
S
A
D
G
K
A
Q
I
N
P
D
F
R
C
D
Y
G
Y
N
I
H
V
G
K
S
F
F
A
N
F
N
C
V
I
L
D
V
C
E
V
R
I
G
D
H
C
M
F
A
P
G
V
H
I
Y
-
T
A
T
H
P
L
H
P
V
E
R
N
S
G
K
E
Y
G
K
P
V
K
I
G
N
N
V
W
V
G
G
G
A
I
I
N
P
G
V
S
I
G
D
N
A
V
I
A
S
G
A
V
V
T
K
D
V
P
N
-
N
V
V
V
G
G
N
P
A
K
V
I
K
T
I
E
E
---------------------------------------------------------------------------------------------
6
PsiBlast_PDB
79.29
52%
-27
- C2 -
-
2IC7
-
?
A:
[1-185]
---
M
K
S
E
K
E
K
M
L
A
G
H
L
Y
N
P
-
A
-
D
L
E
L
V
K
E
R
E
R
A
R
R
L
V
R
L
Y
N
E
--
T
--
L
--
E
T
E
Y
D
K
R
T
G
L
L
K
E
L
F
G
S
T
G
E
-
R
L
F
I
E
P
N
F
R
C
D
Y
G
Y
N
I
H
V
G
E
N
F
F
M
N
F
D
G
V
I
L
D
V
C
E
V
R
I
G
D
H
C
F
I
G
P
G
V
H
I
Y
-
T
A
T
H
P
L
D
P
H
E
R
N
S
G
L
E
Y
G
K
P
V
V
I
G
H
N
V
W
I
G
G
R
A
V
I
N
P
G
V
T
I
G
D
N
A
V
I
A
S
G
A
V
V
T
K
D
V
P
A
-
N
A
V
V
G
G
N
P
A
K
V
I
K
W
L
K
----------------------------------------------------------------------------------------------
7
PsiBlast_PDB
78.64
52%
-30
- C2 -
-
2P2O
-
?
A:
[2-185]
----
K
S
E
K
E
K
M
L
A
G
H
L
Y
N
P
-
A
-
D
L
E
L
V
K
E
R
E
R
A
R
R
L
V
R
L
Y
N
E
--
T
--
L
--
E
T
E
Y
D
K
R
T
G
L
L
K
E
L
F
G
S
T
G
E
-
R
L
F
I
E
P
N
F
R
C
D
Y
G
Y
N
I
H
V
G
E
N
F
F
M
N
F
D
G
V
I
L
D
V
C
E
V
R
I
G
D
H
C
F
I
G
P
G
V
H
I
Y
-
T
A
T
H
P
L
D
P
H
E
R
N
S
G
L
E
Y
G
K
P
V
V
I
G
H
N
V
W
I
G
G
R
A
V
I
N
P
G
V
T
I
G
D
N
A
V
I
A
S
G
A
V
V
T
K
D
V
P
A
-
N
A
V
V
G
G
N
P
A
K
V
I
K
W
L
K
----------------------------------------------------------------------------------------------
144
HHSearch
78.56
54%
-30
- C2 -
-
2P2O
-
?
B:
[2-184]
----
K
S
E
K
E
K
M
L
A
G
H
L
Y
N
P
-
A
-
D
L
E
L
V
K
E
R
E
R
A
R
R
L
V
R
L
Y
N
E
--
T
LE
T
--
E
Y
D
K
--
R
T
G
L
L
K
E
L
F
G
S
T
G
E
-
R
L
F
I
E
P
N
F
R
C
D
Y
G
Y
N
I
H
V
G
E
N
F
F
M
N
F
D
G
V
I
L
D
V
C
E
V
R
I
G
D
H
C
F
I
G
P
G
V
H
I
Y
-
T
A
T
H
P
L
D
P
H
E
R
N
S
G
L
E
Y
G
K
P
V
V
I
G
H
N
V
W
I
G
G
R
A
V
I
N
P
G
V
T
I
G
D
N
A
V
I
A
S
G
A
V
V
T
K
D
V
P
A
-
N
A
V
V
G
G
N
P
A
K
V
I
K
W
L
-----------------------------------------------------------------------------------------------
1
PsiBlast_PDB
78.49
56%
-8
- C2 -
-
3HJJ
10
?
A:
[5-190]
----
K
T
E
K
D
K
M
L
A
G
E
M
Y
I
A
-
D
-
D
E
E
L
V
A
D
R
V
E
A
K
R
L
T
R
L
Y
N
E
--
A
V
E
T
--
G
D
E
R
--
R
F
T
L
L
N
Q
L
L
G
S
S
A
D
G
K
A
Q
I
N
P
D
F
R
C
D
Y
G
Y
N
I
H
V
G
K
S
F
F
A
N
F
N
C
V
I
L
D
V
C
E
V
R
I
G
D
H
C
M
F
A
P
G
V
H
I
Y
-
T
A
T
H
P
L
H
P
V
E
R
N
S
G
K
E
Y
G
K
P
V
K
I
G
N
N
V
W
V
G
G
G
A
I
I
N
P
G
V
S
I
G
D
N
A
V
I
A
S
G
A
V
V
T
K
D
V
P
N
-
N
V
V
V
G
G
N
P
A
K
V
I
K
T
I
E
E
---------------------------------------------------------------------------------------------
143
HHSearch
77.82
35%
-132
- C2 -
-
3NZ2
-
?
J:
[4-187]
--
M
K
M
S
E
L
E
K
M
L
K
G
E
H
F
D
G
-
A
-
S
A
E
I
E
A
L
R
S
Q
A
G
R
L
K
L
E
I
N
Q
--
S
--
L
--
D
E
A
E
--
R
Y
A
L
Q
R
E
L
F
G
H
L
G
H
-
K
S
C
V
Q
P
P
F
H
C
E
F
G
K
T
I
R
I
G
D
H
T
F
I
N
M
N
V
V
M
L
D
G
A
P
I
T
I
G
D
H
V
L
I
G
P
S
T
Q
F
Y
-
T
A
S
H
S
L
D
Y
R
R
R
Q
A
W
E
T
I
C
K
P
I
V
I
E
D
D
V
W
I
G
G
N
V
V
I
N
Q
G
V
T
I
G
A
R
S
V
V
A
A
N
S
V
V
N
Q
D
V
P
P
-
D
T
L
V
G
G
T
P
A
R
I
L
R
S
L
K
----------------------------------------------------------------------------------------------
145
HHSearch
77.56
54%
-30
- C2 -
-
2IC7
-
?
C:
[2-184]
----
K
S
E
K
E
K
M
L
A
G
H
L
Y
N
P
-
A
-
D
L
E
L
V
K
E
R
E
R
A
R
R
L
V
R
L
Y
N
E
--
T
LE
T
--
E
Y
D
K
--
R
T
G
L
L
K
E
L
F
G
S
T
G
E
-
R
L
F
I
E
P
N
F
R
C
D
Y
G
Y
N
I
H
V
G
E
N
F
F
M
N
F
D
G
V
I
L
D
V
C
E
V
R
I
G
D
H
C
F
I
G
P
G
V
H
I
Y
-
T
A
T
H
P
L
D
P
H
E
R
N
S
G
L
E
Y
G
K
P
V
V
I
G
H
N
V
W
I
G
G
R
A
V
I
N
P
G
V
T
I
G
D
N
A
V
I
A
S
G
A
V
V
T
K
D
V
P
A
-
N
A
V
V
G
G
N
P
A
K
V
I
K
W
L
-----------------------------------------------------------------------------------------------
142
HHSearch
76.50
35%
-134
- C2 -
-
3NZ2
1
?
F:
[6-188]
----
M
S
E
L
E
K
M
L
K
G
E
H
F
D
G
-
A
-
S
A
E
I
E
A
L
R
S
Q
A
G
R
L
K
L
E
I
N
Q
--
S
--
L
--
D
E
A
E
--
R
Y
A
L
Q
R
E
L
F
G
H
L
G
H
-
K
S
C
V
Q
P
P
F
H
C
E
F
G
K
T
I
R
I
G
D
H
T
F
I
N
M
N
V
V
M
L
D
G
A
P
I
T
I
G
D
H
V
L
I
G
P
S
T
Q
F
Y
-
T
A
S
H
S
L
D
Y
R
R
R
Q
A
W
E
T
I
C
K
P
I
V
I
E
D
D
V
W
I
G
G
N
V
V
I
N
Q
G
V
T
I
G
A
R
S
V
V
A
A
N
S
V
V
N
Q
D
V
P
P
-
D
T
L
V
G
G
T
P
A
R
I
L
R
S
L
K
D
---------------------------------------------------------------------------------------------
18
PsiBlast_PDB
74.36
35%
-135
- C2 -
-
3NZ2
5
?
A:
[7-187]
-----
S
E
L
E
K
M
L
K
G
E
H
F
D
G
-
A
-
S
A
E
I
E
A
L
R
S
Q
A
G
R
L
K
L
E
I
N
Q
--
S
--
L
--
D
E
A
E
--
R
Y
A
L
Q
R
E
L
F
G
H
L
G
H
-
K
S
C
V
Q
P
P
F
H
C
E
F
G
K
T
I
R
I
G
D
H
T
F
I
N
M
N
V
V
M
L
D
G
A
P
I
T
I
G
D
H
V
L
I
G
P
S
T
Q
F
Y
-
T
A
S
H
S
L
D
Y
R
R
R
Q
A
W
E
T
I
C
K
P
I
V
I
E
D
D
V
W
I
G
G
N
V
V
I
N
Q
G
V
T
I
G
A
R
S
V
V
A
A
N
S
V
V
N
Q
D
V
P
P
-
D
T
L
V
G
G
T
P
A
R
I
L
R
S
L
K
----------------------------------------------------------------------------------------------
14
PsiBlast_PDB
73.72
41%
-8
- C2 -
-
4EGG
3
ATRF2_STAAC
A:
[2-188]
-----
T
E
K
E
K
M
L
A
E
K
W
Y
D
A
-
N
F
D
Q
T
L
I
N
E
R
L
R
A
K
V
I
C
F
A
L
N
H
--
T
--
N
PV
A
T
M
M
--
R
K
V
L
I
D
A
L
F
Q
T
T
T
D
-
N
V
S
I
S
I
P
F
D
T
D
Y
G
W
N
V
K
L
G
K
N
V
Y
V
N
T
N
C
Y
F
M
D
G
G
Q
I
T
I
G
D
N
V
F
I
G
P
N
C
G
F
Y
-
T
A
T
H
P
L
N
F
H
H
R
N
E
G
F
E
K
A
G
P
I
H
I
G
S
N
T
W
F
G
G
H
V
A
V
L
P
G
V
T
I
G
E
G
S
V
I
G
A
G
S
V
V
T
K
D
I
P
P
-
H
S
L
A
V
G
N
P
C
K
V
V
R
K
I
D
N
D
L
-------------------------------------------------------------------------------------------
33
PsiBlast_CBE
72.63
41%
-8
- C2 -
-
4EGG
2
ATRF2_STAAC
D:
[2-188]
-----
T
E
K
E
K
M
L
A
E
K
W
Y
D
A
-
N
F
D
Q
T
L
I
N
E
R
L
R
A
K
V
I
C
F
A
L
N
H
--
T
--
N
PV
A
T
M
M
--
R
K
V
L
I
D
A
L
F
Q
T
T
T
D
-
N
V
S
I
S
I
P
F
D
T
D
Y
G
W
N
V
K
L
G
K
N
V
Y
V
N
T
N
C
Y
F
M
D
G
G
Q
I
T
I
G
D
N
V
F
I
G
P
N
C
G
F
Y
-
T
A
T
H
P
L
N
F
H
H
R
N
E
G
F
E
K
A
G
P
I
H
I
G
S
N
T
W
F
G
G
H
V
A
V
L
P
G
V
T
I
G
E
G
S
V
I
G
A
G
S
V
V
T
K
D
I
P
P
-
H
S
L
A
V
G
N
P
C
K
V
V
R
K
I
D
N
D
L
-------------------------------------------------------------------------------------------
31
PsiBlast_CBE
72.20
41%
-3
- C2 -
-
4EGG
2
ATRF2_STAAC
F:
[2-188]
-----
T
E
K
E
K
M
L
A
E
K
W
Y
D
A
-
N
F
D
Q
T
L
I
N
E
R
L
R
A
K
V
I
C
F
A
L
N
H
--
T
--
N
PV
A
T
M
M
--
R
K
V
L
I
D
A
L
F
Q
T
T
T
D
-
N
V
S
I
S
I
P
F
D
T
D
Y
G
W
N
V
K
L
G
K
N
V
Y
V
N
T
N
C
Y
F
M
D
G
G
Q
I
T
I
G
D
N
V
F
I
G
P
N
C
G
F
Y
-
T
A
T
H
P
L
N
F
H
H
R
N
E
G
F
E
K
A
G
P
I
H
I
G
S
N
T
W
F
G
G
H
V
A
V
L
P
G
V
T
I
G
E
G
S
V
I
G
A
G
S
V
V
T
K
D
I
P
P
-
H
S
L
A
V
G
N
P
C
K
V
V
R
K
I
D
N
D
L
-------------------------------------------------------------------------------------------
23
PsiBlast_CBE
72.12
44%
-28
- C2 -
-
1KRV
4
THGA_ECOLI
C:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
EK
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
34
PsiBlast_CBE
72.09
41%
-2
- C2 -
-
4EGG
2
ATRF2_STAAC
C:
[2-187]
-----
T
E
K
E
K
M
L
A
E
K
W
Y
D
A
-
N
F
D
Q
T
L
I
N
E
R
L
R
A
K
V
I
C
F
A
L
N
H
--
T
--
N
PV
A
T
M
M
--
R
K
V
L
I
D
A
L
F
Q
T
T
T
D
-
N
V
S
I
S
I
P
F
D
T
D
Y
G
W
N
V
K
L
G
K
N
V
Y
V
N
T
N
C
Y
F
M
D
G
G
Q
I
T
I
G
D
N
V
F
I
G
P
N
C
G
F
Y
-
T
A
T
H
P
L
N
F
H
H
R
N
E
G
F
E
K
A
G
P
I
H
I
G
S
N
T
W
F
G
G
H
V
A
V
L
P
G
V
T
I
G
E
G
S
V
I
G
A
G
S
V
V
T
K
D
I
P
P
-
H
S
L
A
V
G
N
P
C
K
V
V
R
K
I
D
N
D
--------------------------------------------------------------------------------------------
11
PsiBlast_PDB
71.13
44%
-28
- C2 -
-
1KRV
4
THGA_ECOLI
A:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
E
K
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
24
PsiBlast_CBE
71.11
44%
-28
- C2 -
-
1KRV
4
THGA_ECOLI
B:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
EK
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
28
PsiBlast_CBE
70.94
44%
-28
- C2 -
-
1KRR
2
THGA_ECOLI
B:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
EK
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
30
PsiBlast_CBE
70.92
44%
-28
- C2 -
-
1KQA
2
THGA_ECOLI
B:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
EK
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
29
PsiBlast_CBE
70.62
44%
-31
- C2 -
-
1KQA
2
THGA_ECOLI
C:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
EK
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
27
PsiBlast_CBE
70.46
44%
-31
- C2 -
-
1KRR
2
THGA_ECOLI
C:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
EK
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
138
HHSearch
70.22
44%
-23
- C2 -
-
1KRU
6
THGA_ECOLI
A:
[2-189]
---
N
M
P
M
T
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
HP
S
--
E
V
E
K
--
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
R
D
-------------------------------------------------------------------------------------------
139
HHSearch
69.93
44%
-23
- C2 -
-
1KRR
2
THGA_ECOLI
A:
[2-189]
---
N
M
P
M
T
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
HP
S
--
E
V
E
K
--
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
R
D
-------------------------------------------------------------------------------------------
10
PsiBlast_PDB
69.81
44%
-29
- C2 -
-
1KRU
6
THGA_ECOLI
A:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
E
K
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
9
PsiBlast_PDB
69.49
44%
-29
- C2 -
-
1KRR
2
THGA_ECOLI
A:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
E
K
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
25
PsiBlast_CBE
69.47
44%
-29
- C2 -
-
1KRU
6
THGA_ECOLI
C:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
EK
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
8
PsiBlast_PDB
69.37
44%
-29
- C2 -
-
1KQA
2
THGA_ECOLI
A:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
E
K
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
141
HHSearch
69.30
42%
4
- C2 -
-
5U2K
6
ATRF2_STAAC
A:
[1-189]
----
M
T
E
K
E
K
M
L
A
E
K
W
Y
D
A
N
F
-
D
Q
Y
L
I
N
E
R
A
R
A
K
D
I
C
F
E
L
N
H
TR
P
--
S
--
A
T
N
K
--
R
K
E
L
I
D
Q
L
F
Q
T
T
T
D
-
N
V
S
I
S
I
P
F
D
T
D
Y
G
W
N
V
K
L
G
K
N
V
Y
V
N
T
N
C
Y
F
M
D
G
G
Q
I
T
I
G
D
N
V
F
I
G
P
N
C
G
F
Y
-
T
A
T
H
P
L
N
F
H
H
R
N
E
G
F
E
K
A
G
P
I
H
I
G
S
N
T
W
F
G
G
H
V
A
V
L
P
G
V
T
I
G
E
G
S
V
I
G
A
G
S
V
V
T
K
D
I
P
P
-
H
S
L
A
V
G
N
P
C
K
V
V
R
K
I
D
N
D
L
P
------------------------------------------------------------------------------------------
146
HHSearch
69.11
42%
1
- C2 -
-
5V0Z
4
ATRF2_STAAC
B:
[4-191]
----
M
T
E
K
E
K
M
L
A
E
K
W
Y
D
A
N
F
-
D
Q
Y
L
I
N
E
R
A
R
A
K
D
I
C
F
E
L
N
H
TR
P
--
S
--
A
T
N
K
--
R
K
E
L
I
D
Q
L
F
Q
T
T
T
D
-
N
V
S
I
S
I
P
F
D
T
D
Y
G
W
N
V
K
L
G
K
N
V
Y
V
N
T
N
C
Y
F
M
D
G
G
Q
I
T
I
G
D
N
V
F
I
G
P
N
C
G
F
Y
-
T
A
T
H
P
L
N
F
H
H
R
N
E
G
F
E
K
A
G
P
I
H
I
G
S
N
T
W
F
G
G
H
V
A
V
L
P
G
V
T
I
G
E
G
S
V
I
G
A
G
S
V
V
T
K
D
I
P
P
-
H
S
L
A
V
G
N
P
C
K
V
V
R
K
I
D
N
D
L
-------------------------------------------------------------------------------------------
26
PsiBlast_CBE
69.01
44%
-25
- C2 -
-
1KRU
6
THGA_ECOLI
B:
[7-187]
--------
E
R
I
R
A
G
K
L
F
T
D
-
M
-
C
E
G
L
P
E
K
R
L
R
G
K
T
L
M
Y
E
F
N
H
--
S
--
H
--
P
S
E
V
EK
R
E
S
L
I
K
E
M
F
A
T
V
G
E
-
N
A
W
V
E
P
P
V
Y
F
S
Y
G
S
N
I
H
I
G
R
N
F
Y
A
N
F
N
L
T
I
V
D
D
Y
T
V
T
I
G
D
N
V
L
I
A
P
N
V
T
L
S
-
V
T
G
H
P
V
H
H
E
L
R
K
N
G
E
M
Y
S
F
P
I
T
I
G
N
N
V
W
I
G
S
H
V
V
I
N
P
G
V
T
I
G
D
N
S
V
I
G
A
G
S
I
V
T
K
D
I
P
P
-
N
V
V
A
A
G
V
P
C
R
V
I
R
E
I
N
D
---------------------------------------------------------------------------------------------
45
PsiBlast_CBE
53.86
42%
-84
- C2 -
-
2WLF
2
OATWY_NEIME
C:
[101-190]
----------------------------------------------------------------------------------------------------------
I
S
I
G
K
D
C
M
L
A
H
G
Y
E
I
R
N
T
D
M
H
P
I
Y
S
L
E
N
G
E
R
I
N
H
G
K
D
V
I
I
G
N
H
V
W
L
G
R
N
V
T
I
L
K
G
V
C
I
P
N
N
V
V
V
G
S
H
T
V
L
Y
K
S
F
K
E
P
N
C
V
I
A
G
S
P
A
K
I
V
K
-------------------------------------------------------------------------------------------------
51
PsiBlast_CBE
53.65
42%
-84
- C2 -
-
2WLD
1
OATWY_NEIME
C:
[101-190]
----------------------------------------------------------------------------------------------------------
I
S
I
G
K
D
C
M
L
A
H
G
Y
E
I
R
N
T
D
M
H
P
I
Y
S
L
E
N
G
E
R
I
N
H
G
K
D
V
I
I
G
N
H
V
W
L
G
R
N
V
T
I
L
K
G
V
C
I
P
N
N
V
V
V
G
S
H
T
V
L
Y
K
S
F
K
E
P
N
C
V
I
A
G
S
P
A
K
I
V
K
-------------------------------------------------------------------------------------------------
42
PsiBlast_CBE
52.41
42%
-84
- C2 -
-
2WLG
6
OATWY_NEIME
C:
[101-190]
----------------------------------------------------------------------------------------------------------
I
S
I
G
K
D
C
M
L
A
H
G
Y
E
I
R
N
T
D
M
H
P
I
Y
S
L
E
N
G
E
R
I
N
H
G
K
D
V
I
I
G
N
H
V
W
L
G
R
N
V
T
I
L
K
G
V
C
I
P
N
N
V
V
V
G
S
H
T
V
L
Y
K
S
F
K
E
P
N
C
V
I
A
G
S
P
A
K
I
V
K
-------------------------------------------------------------------------------------------------
43
PsiBlast_CBE
52.01
42%
-85
- C2 -
-
2WLG
4
OATWY_NEIME
B:
[101-190]
----------------------------------------------------------------------------------------------------------
I
S
I
G
K
D
C
M
L
A
H
G
Y
E
I
R
N
T
D
M
H
P
I
Y
S
L
E
N
G
E
R
I
N
H
G
K
D
V
I
I
G
N
H
V
W
L
G
R
N
V
T
I
L
K
G
V
C
I
P
N
N
V
V
V
G
S
H
T
V
L
Y
K
S
F
K
E
P
N
C
V
I
A
G
S
P
A
K
I
V
K
-------------------------------------------------------------------------------------------------
47
PsiBlast_CBE
51.83
42%
-89
- C2 -
-
2WLF
4
OATWY_NEIME
A:
[101-190]
----------------------------------------------------------------------------------------------------------
I
S
I
G
K
D
C
M
L
A
H
G
Y
E
I
R
N
T
D
M
H
P
I
Y
S
L
E
N
G
E
R
I
N
H
G
K
D
V
I
I
G
N
H
V
W
L
G
R
N
V
T
I
L
K
G
V
C
I
P
N
N
V
V
V
G
S
H
T
V
L
Y
K
S
F
K
E
P
N
C
V
I
A
G
S
P
A
K
I
V
K
-------------------------------------------------------------------------------------------------
44
PsiBlast_CBE
51.72
42%
-88
- C2 -
-
2WLG
5
OATWY_NEIME
A:
[101-190]
----------------------------------------------------------------------------------------------------------
I
S
I
G
K
D
C
M
L
A
H
G
Y
E
I
R
N
T
D
M
H
P
I
Y
S
L
E
N
G
E
R
I
N
H
G
K
D
V
I
I
G
N
H
V
W
L
G
R
N
V
T
I
L
K
G
V
C
I
P
N
N
V
V
V
G
S
H
T
V
L
Y
K
S
F
K
E
P
N
C
V
I
A
G
S
P
A
K
I
V
K
-------------------------------------------------------------------------------------------------
49
PsiBlast_CBE
51.66
42%
-84
- C2 -
-
2WLE
1
OATWY_NEIME
B:
[101-190]
----------------------------------------------------------------------------------------------------------
I
S
I
G
K
D
C
M
L
A
H
G
Y
E
I
R
N
T
D
M
H
P
I
Y
S
L
E
N
G
E
R
I
N
H
G
K
D
V
I
I
G
N
H
V
W
L
G
R
N
V
T
I
L
K
G
V
C
I
P
N
N
V
V
V
G
S
H
T
V
L
Y
K
S
F
K
E
P
N
C
V
I
A
G
S
P
A
K
I
V
K
-------------------------------------------------------------------------------------------------
48
PsiBlast_CBE
51.53
42%
-83
- C2 -
-
2WLE
2
OATWY_NEIME
C:
[101-190]
----------------------------------------------------------------------------------------------------------
I
S
I
G
K
D
C
M
L
A
H
G
Y
E
I
R
N
T
D
M
H
P
I
Y
S
L
E
N
G
E
R
I
N
H
G
K
D
V
I
I
G
N
H
V
W
L
G
R
N
V
T
I
L
K
G
V
C
I
P
N
N
V
V
V
G
S
H
T
V
L
Y
K
S
F
K
E
P
N
C
V
I
A
G
S
P
A
K
I
V
K
-------------------------------------------------------------------------------------------------
53
PsiBlast_CBE
51.43
42%
-82
- C2 -
-
2WLD
2
OATWY_NEIME
A:
[101-190]
----------------------------------------------------------------------------------------------------------
I
S
I
G
K
D
C
M
L
A
H
G
Y
E
I
R
N
T
D
M
H
P
I
Y
S
L
E
N
G
E
R
I
N
H
G
K
D
V
I
I
G
N
H
V
W
L
G
R
N
V
T
I
L
K
G
V
C
I
P
N
N
V
V
V
G
S
H
T
V
L
Y
K
S
F
K
E
P
N
C
V
I
A
G
S
P
A
K
I
V
K
-------------------------------------------------------------------------------------------------
46
PsiBlast_CBE
50.65
42%
-86
- C2 -
-
2WLF
3
OATWY_NEIME
B:
[101-190]
----------------------------------------------------------------------------------------------------------
I
S
I
G
K
D
C
M
L
A
H
G
Y
E
I
R
N
T
D
M
H
P
I
Y
S
L
E
N
G
E
R
I
N
H
G
K
D
V
I
I
G
N
H
V
W
L
G
R
N
V
T
I
L
K
G
V
C
I
P
N
N
V
V
V
G
S
H
T
V
L
Y
K
S
F
K
E
P
N
C
V
I
A
G
S
P
A
K
I
V
K
-------------------------------------------------------------------------------------------------