@TOME V2.3
(Mar 2018)
Ref.
-
-
Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : Q3XXL7: (2018-01-04 )
MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKASQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGMVKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDEEAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVDPLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKKHGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSALTKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPMDHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQETGIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILEAEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELVSDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLALTVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPNSLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNVHVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGHHTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIESLKQINLLEMTPMDALNTLYELQKQL
Atome Classification :
(27 SA)
.........10........20........30........40........50........60........70........80........90........100.......110.......120.......130.......140.......150.......160.......170.......180.--...---.-..190.......200..---.....210..-----....------.220........230.......240.......250.......260.......270.......280.......290.......300.......310.......320.......330.......340.......350.......360.......370.......380.......390.......400.......410.......420.......430.......440.......450.......460.......470.......480.......490.......500.......510.......520.......530.......540.......550.......560.......570.......580.......590.......600.......610.......620.......630.......640.......650.......660.......670.......680.......690.......700.......710.......720.......730.......740.......750.......760.......770.......780.......790.......800.......810.......820.......830.......840.......850.......860.......870.......880
(Atome)
(Ident)
(Tito)
(Num)
(QMean)
(Ligand)
(Uniprot)
MPQKTKN
T
P
M
M
E
Q
Y
L
S
I
K
A
QYQ
D
A
F
L
F
Y
R
L
G
D
F
Y
E
L
F
Y
E
DAI
K
A
S
Q
L
L
E
L
T
L
TSRNRNAEEPIPMCGVP
H
H
A
A
Q
G
Y
I
D
A
L
I
EKG
Y
K
V
A
I
C
EQVEDPKT
T
K
G
M
V
K
R
E
V
I
Q
LVTPGTVMDSKGLSAKDN
N
F
L
T
A
VVSQGNEF
G
F
A
Y
A
DLSTGE
L
K
T
A
RLH
D
E
E
A
V
L
N
E
A
S
AL
Q
T
K
E
L
VLG
S
E
I
T
D
--
T
L
R
---
E
-
Q
LSGRL
G
L
V
FSQQNE
M
E
---ENAE
F
S
F
L
T
S
-----
E
L
V
D
------
P
L
E
-
K
EATG
K
L
L
T
Y
L
S
V
T
Q
K
R
S
L
A
H
I
Q
K
A
VEYQPD
H
F
L
K
MD
Y
Y
S
KFNL
E
L
S
Q
S
IRTGKKH
G
T
L
L
W
L
LDET
K
T
AM
G
G
R
L
L
K
Q
W
L
DRP
L
I
Q
A
K
Q
I
K
A
R
Q
E
M
V
A
S
L
L
D
S
Y
F
E
R
I
D
L
Q
S
A
L
T
K
V
Y
D
L
E
R
L
AG
R
V
A
FGNVNG
R
D
L
I
Q
L
K
T
S
L
E
Q
V
P
M
I
R
G
L
I
EGID
Q
G
Q
W
Q
S
L
L
S
EMQ
P
M
D
H
L
V
Q
L
I
D
Q
A
IQDDPP
L
Q
ITEGNIIKD
G
F
N
E
Q
L
D
M
Y
R
S
A
M
R
N
G
K
K
W
L
A
E
L
E
A
K
E
R
Q
E
TGIKN
L
K
I
G
F
N
R
V
F
G
Y
Y
I
E
I
T
KANLG
N
A
E
L
E
K
Y
E
R
K
Q
T
L
A
N
A
E
RFIT
P
E
L
K
E
L
E
T
Q
I
L
E
AEEKSV
D
L
E
Y
Q
L
F
L
A
V
R
E
E
V
K
K
A
I
Q
P
L
Q
V
L
A
K
A
I
S
A
V
D
V
L
Q
S
F
A
T
I
S
E
R
Y
Q
YV
R
P
E
L
VSD
K
H
Q
L
L
IKDGR
H
P
V
V
E
K
V
LG
H
Q
E
YIPNSVEMAED
E
M
I
L
L
I
TGPNMS
G
K
S
T
Y
M
R
Q
L
A
L
T
V
L
M
A
QMGCFV
P
A
K
Q
A
V
L
P
I
F
D
R
I
F
T
RIGASDDLIAGQ
S
T
F
M
V
E
M
M
EANQ
A
L
RYATPN
S
L
I
L
F
DELGRGTATY
D
G
M
A
L
A
Q
A
I
I
E
Y
I
H
R
H
V
Q
A
K
T
L
F
S
T
H
Y
H
E
L
T
V
L
E
K
E
L
P
Q
LKNVHV
G
A
VEKDG
E
V
V
F
L
H
K
MMDGPADKSYG
I
H
V
A
K
I
AGL
P
S
N
L
L
E
R
A
A
D
I
L
H
T
LENGDNGHHTV
P
A
E
V
E
E
K
TKTA
K
Q
EQIKP
P
A
E
KV
N
Q
D
H
I
Q
D
R
V
K
E
E
N
Q
Q
L
S
L
F
S
E
LSE
N
E
T
E
V
I
E
S
L
K
QIN
L
L
EMT
P
M
D
A
L
N
T
L
Y
E
L
Q
KQL
39
HHSearch
94.55
40%
-93
- C4 -
-
1EWQ
3
MUTS_THEAQ
A:
[9-763]
--
G
P
G
P
L
P
P
L
L
Q
Q
Y
V
E
L
R
D
Q
Y
P
D
Y
L
L
L
F
Q
V
G
D
F
Y
E
C
F
G
E
D
A
E
R
L
A
R
A
L
G
L
V
L
T
H
K
T
S
-
K
D
F
T
T
P
M
A
G
I
P
L
R
A
F
E
A
Y
A
E
R
L
L
K
M
G
F
R
L
A
V
A
D
Q
V
E
P
A
E
E
A
E
G
L
V
R
R
E
V
T
Q
L
L
T
P
G
T
L
L
Q
E
S
L
L
P
-
R
E
A
N
Y
L
A
A
I
A
T
G
D
-
G
W
G
L
A
F
L
D
V
S
T
G
E
F
K
G
T
V
L
K
S
K
S
A
L
Y
D
E
L
F
R
H
R
P
A
E
V
L
L
A
P
E
L
L
E
--
N
G
A
-----
F
L
D
E
F
R
-
K
R
F
P
V
M
--
L
-----
S
E
A
P
F
E
P
E
G
------
E
G
P
------
L
A
L
-
R
R
A
R
G
A
L
L
A
Y
A
Q
R
T
Q
G
G
A
L
-
S
L
Q
P
F
R
F
Y
D
P
G
A
F
M
R
L
P
E
A
T
L
R
A
L
E
V
F
E
P
L
---
R
G
Q
D
T
L
F
S
V
L
D
E
T
R
T
A
P
G
R
R
L
L
Q
S
W
L
R
H
P
L
L
D
R
G
P
L
E
A
R
L
D
R
V
E
G
F
V
R
E
G
A
L
R
E
G
V
R
R
L
L
Y
R
L
A
D
L
E
R
L
A
T
R
L
E
L
G
R
A
S
P
K
D
L
G
A
L
R
R
S
L
Q
I
L
P
E
L
R
A
L
L
G
E
E
V
---------
G
L
P
D
L
S
P
L
K
E
E
L
E
A
A
L
V
E
D
P
P
L
K
V
S
E
G
G
L
I
R
E
G
Y
D
P
D
L
D
A
L
R
A
A
H
R
E
G
V
A
Y
F
L
E
L
E
E
R
E
R
E
R
T
G
I
P
T
L
K
V
G
Y
N
A
V
F
G
Y
Y
L
E
V
T
R
P
Y
Y
E
R
V
P
K
E
-
Y
R
P
V
Q
T
L
K
D
R
Q
R
Y
T
L
P
E
M
K
E
K
E
R
E
V
Y
R
L
E
A
L
I
R
R
R
E
E
E
V
F
L
E
V
R
E
R
A
K
R
Q
A
E
A
L
R
E
A
A
R
I
L
A
E
L
D
V
Y
A
A
L
A
E
V
A
V
R
Y
G
Y
V
R
P
R
F
G
---
D
R
L
Q
I
R
A
G
R
H
P
V
V
E
R
R
---
T
E
F
V
P
N
D
L
E
M
A
--
H
E
L
V
L
I
T
G
P
N
M
A
G
K
S
T
F
L
R
Q
T
A
L
I
A
L
L
A
Q
V
G
S
F
V
P
A
E
E
A
H
L
P
L
F
D
G
I
Y
T
R
I
G
A
------
G
K
S
T
F
M
V
E
M
E
E
V
A
L
I
L
K
E
A
T
E
N
S
L
V
L
L
D
E
V
G
R
G
T
S
S
L
D
G
V
A
I
A
T
A
V
A
E
A
L
H
E
R
-
R
A
Y
T
L
F
A
T
H
Y
F
E
L
T
A
L
G
L
--
P
R
L
K
N
L
H
V
A
A
R
E
E
A
G
G
L
V
F
Y
H
Q
V
L
P
G
P
A
S
K
S
Y
G
V
E
V
A
A
M
A
G
L
P
K
E
V
V
A
R
A
R
A
L
L
Q
A
M
A
-----------------------------------------------------------------------------------------
12
PsiBlast_PDB
86.43
39%
-19
- C4 -
-
1NNE
4
MUTS_THEAQ
A:
[15-762]
--------
P
L
L
Q
Q
Y
V
E
L
R
D
Q
Y
P
D
Y
L
L
L
F
Q
V
G
D
F
Y
E
C
F
G
E
D
A
E
R
L
A
R
A
L
G
L
V
L
T
H
K
T
-
S
K
D
F
T
T
P
M
A
G
I
P
L
R
A
F
E
A
Y
A
E
R
L
L
K
M
G
F
R
L
A
V
A
D
Q
V
E
P
A
E
E
A
E
G
L
V
R
R
E
V
T
Q
L
L
T
P
G
T
L
L
-
Q
E
S
L
L
P
R
E
A
N
Y
L
A
A
I
A
T
-
G
D
G
W
G
L
A
F
L
D
V
S
T
G
E
F
K
G
T
V
L
K
S
K
S
A
L
Y
D
E
L
F
R
H
R
P
A
E
V
L
L
A
P
E
L
L
E
N
G
A
F
L
---
D
-
E
F
R
K
R
F
P
V
M
L
S
E
A
P
-
F
E
---
P
E
G
E
--------------
G
------
P
L
A
L
R
R
A
R
G
A
L
L
A
Y
A
Q
R
T
Q
G
G
A
L
S
-
L
Q
P
F
R
F
Y
D
P
G
A
F
M
R
L
P
E
A
T
L
R
A
L
E
V
F
E
P
L
R
G
---
Q
D
T
L
F
S
V
L
D
E
T
R
T
A
P
G
R
R
L
L
Q
S
W
L
R
H
P
L
L
D
R
G
P
L
E
A
R
L
D
R
V
E
G
F
V
R
E
G
A
L
R
E
G
V
R
R
L
L
Y
R
L
A
D
L
E
R
L
A
T
R
L
E
L
G
R
A
S
P
K
D
L
G
A
L
R
R
S
L
Q
I
L
P
E
L
R
A
L
L
-
G
E
E
V
G
-----
L
P
D
L
S
P
L
K
---
E
E
L
E
A
A
L
V
E
D
P
P
L
K
V
S
E
G
G
L
I
R
E
G
Y
D
P
D
L
D
A
L
R
A
A
H
R
E
G
V
A
Y
F
L
E
L
E
E
R
E
R
E
R
T
G
I
P
T
L
K
V
G
Y
N
A
V
F
G
Y
Y
L
E
V
T
R
P
Y
Y
E
R
V
P
K
E
-
Y
R
P
V
Q
T
L
K
D
R
Q
R
Y
T
L
P
E
M
K
E
K
E
R
E
V
Y
R
L
E
A
L
I
R
R
R
E
E
E
V
F
L
E
V
R
E
R
A
K
R
Q
A
E
A
L
R
E
A
A
R
I
L
A
E
L
D
V
Y
A
A
L
A
E
V
A
V
R
Y
G
Y
V
R
P
R
F
-
G
D
R
L
Q
--
I
R
A
G
R
H
P
V
V
E
R
---
R
T
E
F
V
P
N
D
L
E
M
A
H
E
--
L
V
L
I
T
G
P
N
M
A
G
K
S
T
F
L
R
Q
T
A
L
I
A
L
L
A
Q
V
G
S
F
V
P
A
E
E
A
H
L
P
L
F
D
G
I
Y
T
R
I
G
A
------
G
K
S
T
F
M
V
E
M
E
E
V
A
L
I
L
K
E
A
T
E
N
S
L
V
L
L
D
E
V
G
R
G
T
S
S
L
D
G
V
A
I
A
T
A
V
A
E
A
L
H
E
R
-
R
A
Y
T
L
F
A
T
H
Y
F
E
L
T
A
L
--
G
L
P
R
L
K
N
L
H
V
A
A
R
E
E
A
G
G
L
V
F
Y
H
Q
V
L
P
G
P
A
S
K
S
Y
G
V
E
V
A
A
M
A
G
L
P
K
E
V
V
A
R
A
R
A
L
L
Q
A
M
------------------------------------------------------------------------------------------
13
PsiBlast_PDB
85.60
39%
-20
- C4 -
-
1FW6
3
MUTS_THEAQ
A:
[15-752]
--------
P
L
L
Q
Q
Y
V
E
L
R
D
Q
Y
P
D
Y
L
L
L
F
Q
V
G
D
F
Y
E
C
F
G
E
D
A
E
R
L
A
R
A
L
G
L
V
L
T
H
K
T
-
S
K
D
F
T
T
P
M
A
G
I
P
L
R
A
F
E
A
Y
A
E
R
L
L
K
M
G
F
R
L
A
V
A
D
Q
V
E
P
A
E
E
A
E
G
L
V
R
R
E
V
T
Q
L
L
T
P
G
T
L
L
-
Q
E
S
L
L
P
R
E
A
N
Y
L
A
A
I
A
T
-
G
D
G
W
G
L
A
F
L
D
V
S
T
G
E
F
K
G
T
V
L
K
S
K
S
A
L
Y
D
E
L
F
R
H
R
P
A
E
V
L
L
A
P
E
L
L
E
N
G
A
F
L
---
D
-
E
F
R
K
R
F
P
V
M
L
S
E
A
P
-
F
E
---
P
E
G
E
--------------
G
------
P
L
A
L
R
R
A
R
G
A
L
L
A
Y
A
Q
R
T
Q
G
G
A
L
S
-
L
Q
P
F
R
F
Y
D
P
G
A
F
M
R
L
P
E
A
T
L
R
A
L
E
V
F
E
P
L
R
G
---
Q
D
T
L
F
S
V
L
D
E
T
R
T
A
P
G
R
R
L
L
Q
S
W
L
R
H
P
L
L
D
R
G
P
L
E
A
R
L
D
R
V
E
G
F
V
R
E
G
A
L
R
E
G
V
R
R
L
L
Y
R
L
A
D
L
E
R
L
A
T
R
L
E
L
G
R
A
S
P
K
D
L
G
A
L
R
R
S
L
Q
I
L
P
E
L
R
A
L
L
-
G
E
E
V
G
-----
L
P
D
L
S
P
L
K
---
E
E
L
E
A
A
L
V
E
D
P
P
L
K
V
S
E
G
G
L
I
R
E
G
Y
D
P
D
L
D
A
L
R
A
A
H
R
E
G
V
A
Y
F
L
E
L
E
E
R
E
R
E
R
T
G
I
P
T
L
K
V
G
Y
N
A
V
F
G
Y
Y
L
E
V
T
R
P
Y
Y
E
R
V
P
K
E
-
Y
R
P
V
Q
T
L
K
D
R
Q
R
Y
T
L
P
E
M
K
E
K
E
R
E
V
Y
R
L
E
A
L
I
R
R
R
E
E
E
V
F
L
E
V
R
E
R
A
K
R
Q
A
E
A
L
R
E
A
A
R
I
L
A
E
L
D
V
Y
A
A
L
A
E
V
A
V
R
Y
G
Y
V
R
P
R
F
-
G
D
R
L
Q
--
I
R
A
G
R
H
P
V
V
E
R
---
R
T
E
F
V
P
N
D
L
E
M
A
H
E
--
L
V
L
I
T
G
P
N
M
A
G
K
S
T
F
L
R
Q
T
A
L
I
A
L
L
A
Q
V
G
S
F
V
P
A
E
E
A
H
L
P
L
F
D
G
I
Y
T
R
I
G
A
------
G
K
S
T
F
M
V
E
M
E
E
V
A
L
I
L
K
E
A
T
E
N
S
L
V
L
L
D
E
V
G
R
G
T
S
S
L
D
G
V
A
I
A
T
A
V
A
E
A
L
H
E
R
-
R
A
Y
T
L
F
A
T
H
Y
F
E
L
T
A
L
--
G
L
P
R
L
K
N
L
H
V
A
A
R
E
E
A
G
G
L
V
F
Y
H
Q
V
L
P
G
P
A
S
K
S
Y
G
V
E
V
A
A
M
A
G
L
P
K
E
V
V
----------------------------------------------------------------------------------------------------
14
PsiBlast_PDB
84.80
39%
-18
- C4 -
-
1EWQ
3
MUTS_THEAQ
A:
[15-752]
--------
P
L
L
Q
Q
Y
V
E
L
R
D
Q
Y
P
D
Y
L
L
L
F
Q
V
G
D
F
Y
E
C
F
G
E
D
A
E
R
L
A
R
A
L
G
L
V
L
T
H
K
T
-
S
K
D
F
T
T
P
M
A
G
I
P
L
R
A
F
E
A
Y
A
E
R
L
L
K
M
G
F
R
L
A
V
A
D
Q
V
E
P
A
E
E
A
E
G
L
V
R
R
E
V
T
Q
L
L
T
P
G
T
L
L
-
Q
E
S
L
L
P
R
E
A
N
Y
L
A
A
I
A
T
-
G
D
G
W
G
L
A
F
L
D
V
S
T
G
E
F
K
G
T
V
L
K
S
K
S
A
L
Y
D
E
L
F
R
H
R
P
A
E
V
L
L
A
P
E
L
L
E
N
G
A
F
L
---
D
-
E
F
R
K
R
F
P
V
M
L
S
E
A
P
-
F
E
---
P
E
G
E
--------------
G
------
P
L
A
L
R
R
A
R
G
A
L
L
A
Y
A
Q
R
T
Q
G
G
A
L
S
-
L
Q
P
F
R
F
Y
D
P
G
A
F
M
R
L
P
E
A
T
L
R
A
L
E
V
F
E
P
L
R
G
---
Q
D
T
L
F
S
V
L
D
E
T
R
T
A
P
G
R
R
L
L
Q
S
W
L
R
H
P
L
L
D
R
G
P
L
E
A
R
L
D
R
V
E
G
F
V
R
E
G
A
L
R
E
G
V
R
R
L
L
Y
R
L
A
D
L
E
R
L
A
T
R
L
E
L
G
R
A
S
P
K
D
L
G
A
L
R
R
S
L
Q
I
L
P
E
L
R
A
L
L
-
G
E
E
V
G
-----
L
P
D
L
S
P
L
K
---
E
E
L
E
A
A
L
V
E
D
P
P
L
K
V
S
E
G
G
L
I
R
E
G
Y
D
P
D
L
D
A
L
R
A
A
H
R
E
G
V
A
Y
F
L
E
L
E
E
R
E
R
E
R
T
G
I
P
T
L
K
V
G
Y
N
A
V
F
G
Y
Y
L
E
V
T
R
P
Y
Y
E
R
V
P
K
E
-
Y
R
P
V
Q
T
L
K
D
R
Q
R
Y
T
L
P
E
M
K
E
K
E
R
E
V
Y
R
L
E
A
L
I
R
R
R
E
E
E
V
F
L
E
V
R
E
R
A
K
R
Q
A
E
A
L
R
E
A
A
R
I
L
A
E
L
D
V
Y
A
A
L
A
E
V
A
V
R
Y
G
Y
V
R
P
R
F
-
G
D
R
L
Q
--
I
R
A
G
R
H
P
V
V
E
R
---
R
T
E
F
V
P
N
D
L
E
M
A
H
E
--
L
V
L
I
T
G
P
N
M
A
G
K
S
T
F
L
R
Q
T
A
L
I
A
L
L
A
Q
V
G
S
F
V
P
A
E
E
A
H
L
P
L
F
D
G
I
Y
T
R
I
G
A
------
G
K
S
T
F
M
V
E
M
E
E
V
A
L
I
L
K
E
A
T
E
N
S
L
V
L
L
D
E
V
G
R
G
T
S
S
L
D
G
V
A
I
A
T
A
V
A
E
A
L
H
E
R
-
R
A
Y
T
L
F
A
T
H
Y
F
E
L
T
A
L
--
G
L
P
R
L
K
N
L
H
V
A
A
R
E
E
A
G
G
L
V
F
Y
H
Q
V
L
P
G
P
A
S
K
S
Y
G
V
E
V
A
A
M
A
G
L
P
K
E
V
V
----------------------------------------------------------------------------------------------------
8
PsiBlast_PDB
84.43
40%
1
- C4 -
-
3K0S
3
MUTS_ECOLI
A:
[9-797]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
------------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
N
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
7
PsiBlast_PDB
84.23
40%
-3
- C4 -
-
1WBD
3
MUTS_ECOLI
A:
[10-798]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
Q
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
10
PsiBlast_PDB
83.97
40%
-1
- C4 -
-
1WB9
3
MUTS_ECOLI
A:
[10-798]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
T
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
2
PsiBlast_PDB
83.62
40%
-1
- C4 -
-
1OH6
3
MUTS_ECOLI
A:
[10-798]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
35
HHSearch
83.40
40%
1
- C4 -
-
1WB9
3
MUTS_ECOLI
A:
[6-798]
--
N
F
D
A
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
T
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
IEG
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
---
Q
L
N
L
Q
F
G
T
R
D
LVGFG
V
E
N
A
------
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
-
G
G
A
E
N
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
N
-
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
D
K
-
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
11
PsiBlast_PDB
83.14
39%
1
- C4 -
-
1E3M
3
MUTS_ECOLI
A:
[10-798]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
5
PsiBlast_PDB
83.14
40%
-3
- C4 -
-
2WTU
1
MUTS_ECOLI
A:
[10-798]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
A
A
D
--------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
29
PsiBlast_CBE
83.12
39%
-18
* C4 *
-
1NNE
5
MUTS_THEAQ
B:
[15-762]
--------
P
L
L
Q
Q
Y
V
E
L
R
D
Q
Y
P
D
Y
L
L
L
F
Q
V
G
D
F
Y
E
C
F
G
E
D
A
E
R
L
A
R
A
L
G
L
V
L
T
H
K
T
-
S
K
D
F
T
T
P
M
A
G
I
P
L
R
A
F
E
A
Y
A
E
R
L
L
K
M
G
F
R
L
A
V
A
D
Q
V
E
P
------
L
V
R
R
E
V
T
Q
L
L
T
P
G
T
L
L
-
Q
E
S
L
L
P
R
E
A
N
Y
L
A
A
I
A
T
-
G
D
G
W
G
L
A
F
L
D
V
S
T
G
E
F
K
G
T
V
L
K
S
K
S
A
L
Y
D
E
L
F
R
H
R
P
A
E
V
L
L
A
P
E
L
L
E
N
G
A
F
L
---
D
-
E
F
R
K
R
F
P
V
M
L
S
E
A
P
-
F
E
---
P
E
G
E
--------------
G
------
P
L
A
L
R
R
A
R
G
A
L
L
A
Y
A
Q
R
T
Q
G
G
A
L
S
-
L
Q
P
F
R
F
Y
D
P
G
A
F
M
R
L
P
E
A
T
L
R
A
L
E
V
F
E
P
L
R
G
---
Q
D
T
L
F
S
V
L
D
E
T
R
T
A
P
G
R
R
L
L
Q
S
W
L
R
H
P
L
L
D
R
G
P
L
E
A
R
L
D
R
V
E
G
F
V
R
E
G
A
L
R
E
G
V
R
R
L
L
Y
R
L
A
D
L
E
R
L
A
T
R
L
E
L
G
R
A
S
P
K
D
L
G
A
L
R
R
S
L
Q
I
L
P
E
L
R
A
L
L
-
G
E
E
V
G
-----
L
P
D
L
S
P
L
K
---
E
E
L
E
A
A
L
V
E
D
P
P
L
K
V
S
E
G
G
L
I
R
E
G
Y
D
P
D
L
D
A
L
R
A
A
H
R
E
G
V
A
Y
F
L
E
L
E
E
R
E
R
E
R
T
G
I
P
T
L
K
V
G
Y
N
A
V
F
G
Y
Y
L
E
V
T
R
P
Y
Y
E
R
V
P
K
E
-
Y
R
P
V
Q
T
L
K
D
R
Q
R
Y
T
L
P
E
M
K
E
K
E
R
E
V
Y
R
L
E
A
L
I
R
R
R
E
E
E
V
F
L
E
V
R
E
R
A
K
R
Q
A
E
A
L
R
E
A
A
R
I
L
A
E
L
D
V
Y
A
A
L
A
E
V
A
V
R
Y
G
Y
V
R
P
R
F
-
G
D
R
L
Q
--
I
R
A
G
R
H
P
V
V
E
R
---
R
T
E
F
V
P
N
D
L
E
M
A
H
E
--
L
V
L
I
T
G
P
N
M
A
G
K
S
T
F
L
R
Q
T
A
L
I
A
L
L
A
Q
V
G
S
F
V
P
A
E
E
A
H
L
P
L
F
D
G
I
Y
T
R
I
G
A
S
D
D
L
A
G
G
K
S
T
F
M
V
E
M
E
E
V
A
L
I
L
K
E
A
T
E
N
S
L
V
L
L
D
E
V
G
R
G
T
S
S
L
D
G
V
A
I
A
T
A
V
A
E
A
L
H
E
R
-
R
A
Y
T
L
F
A
T
H
Y
F
E
L
T
A
L
--
G
L
P
R
L
K
N
L
H
V
A
A
R
E
E
A
G
G
L
V
F
Y
H
Q
V
L
P
G
P
A
S
K
S
Y
G
V
E
V
A
A
M
A
G
L
P
K
E
V
V
A
R
A
R
A
L
L
Q
A
M
------------------------------------------------------------------------------------------
3
PsiBlast_PDB
82.88
40%
-0
- C4 -
-
1OH7
3
MUTS_ECOLI
A:
[10-798]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
1
PsiBlast_PDB
82.61
40%
-1
- C4 -
-
1OH5
3
MUTS_ECOLI
A:
[10-798]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
9
PsiBlast_PDB
82.47
40%
0
- C4 -
-
1WBB
3
MUTS_ECOLI
A:
[10-798]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
A
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
6
PsiBlast_PDB
82.39
40%
3
- C4 -
-
3ZLJ
2
MUTS_ECOLI
A:
[10-798]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
------------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
---------
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
25
PsiBlast_CBE
82.35
40%
1
- C4 -
-
1WBD
2
MUTS_ECOLI
B:
[14-798]
----------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
Q
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
4
PsiBlast_PDB
82.18
40%
2
- C4 -
-
1OH8
3
MUTS_ECOLI
A:
[10-798]
------
H
T
P
M
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
K
R
G
A
S
A
G
E
P
I
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
I
G
D
P
A
T
S
K
G
P
V
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
-----------
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
20
PsiBlast_CBE
81.95
40%
4
- C4 -
-
1W7A
4
MUTS_ECOLI
B:
[14-798]
----------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
A
A
D
D
--
S
G
R
S
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
28
PsiBlast_CBE
81.94
40%
1
- C4 -
-
1WB9
2
MUTS_ECOLI
B:
[14-798]
----------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
T
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
27
PsiBlast_CBE
81.69
40%
1
- C4 -
-
1WBB
2
MUTS_ECOLI
B:
[14-798]
----------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
A
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
22
PsiBlast_CBE
81.58
40%
1
- C4 -
-
1OH7
2
MUTS_ECOLI
B:
[14-798]
----------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
21
PsiBlast_CBE
80.91
40%
2
- C4 -
-
1OH8
2
MUTS_ECOLI
B:
[14-798]
----------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
23
PsiBlast_CBE
80.86
40%
3
- C4 -
-
1OH6
2
MUTS_ECOLI
B:
[14-798]
----------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
24
PsiBlast_CBE
80.76
40%
2
- C4 -
-
1OH5
2
MUTS_ECOLI
B:
[14-798]
----------
M
Q
Q
Y
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
-------------
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
----------
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
26
PsiBlast_CBE
80.66
40%
5
- C4 -
-
3K0S
2
MUTS_ECOLI
B:
[17-797]
--------------
L
R
L
K
A
Q
H
P
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
L
T
----------
P
M
A
G
I
P
Y
H
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
Q
-----------
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
---------
S
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
N
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------
18
PsiBlast_CBE
78.18
40%
12
- C4 -
-
3ZLJ
2
MUTS_ECOLI
B:
[26-798]
----------------------
E
I
L
L
F
Y
R
M
G
D
F
Y
E
L
F
Y
D
D
A
K
R
A
S
Q
L
L
D
I
S
--------------------
A
V
E
N
Y
L
A
K
L
V
N
Q
G
E
S
V
A
I
C
E
------------
E
R
K
V
V
R
I
V
T
P
G
T
I
S
D
E
A
L
L
Q
E
R
Q
D
N
L
L
A
A
I
W
Q
D
S
K
G
F
G
Y
A
T
L
D
I
S
S
G
R
F
R
L
S
E
P
A
D
R
E
T
M
A
A
E
L
Q
R
T
N
P
A
E
L
L
Y
A
E
D
F
A
E
--
M
S
L
---
I
-
E
G
R
R
G
L
R
R
R
P
L
W
E
F
E
I
D
T
A
R
Q
Q
L
N
L
Q
F
G
T
R
-----
D
L
V
G
FGVENA
P
R
G
-
L
C
A
A
G
C
L
L
Q
Y
A
K
D
T
Q
R
T
T
L
P
H
I
R
S
I
T
M
E
R
E
Q
D
S
I
I
M
D
A
A
T
R
R
N
L
E
I
T
Q
N
L
A
G
G
A
E
N
-
T
L
A
S
V
L
D
C
T
V
T
P
M
G
S
R
M
L
K
R
W
L
H
M
P
V
R
D
T
R
V
L
L
E
R
Q
Q
T
I
G
A
L
Q
D
F
---
T
A
G
L
Q
P
V
L
R
Q
V
G
D
L
E
R
I
L
A
R
L
A
L
R
T
A
R
P
R
D
L
A
R
M
R
H
A
F
Q
Q
L
P
E
L
R
A
Q
L
E
T
V
D
S
A
P
V
Q
A
L
R
E
K
M
G
E
F
A
E
L
R
D
L
L
E
R
A
I
I
D
T
P
P
V
L
V
R
D
G
G
V
I
A
S
G
Y
N
E
E
L
D
E
W
R
A
L
A
D
G
A
T
D
Y
L
E
R
L
E
V
R
E
R
E
R
T
G
L
D
T
L
K
V
G
F
N
A
V
H
G
Y
Y
I
Q
I
S
R
G
Q
S
H
L
A
P
I
-
N
Y
M
R
R
Q
T
L
K
N
A
E
R
Y
I
I
P
E
L
K
E
Y
E
D
K
V
L
T
S
K
G
K
A
L
A
L
E
K
Q
L
Y
E
E
L
F
D
L
L
L
P
H
L
E
A
L
Q
Q
S
A
S
A
L
A
E
L
D
V
L
V
N
L
A
E
R
A
Y
T
L
N
Y
T
C
P
T
F
I
-
D
K
P
G
I
R
I
T
E
G
R
H
P
V
V
E
Q
V
L
-
N
E
P
F
I
A
N
P
L
N
L
S
P
Q
R
R
M
L
I
I
T
G
P
N
M
G
G
K
S
T
Y
M
R
Q
T
A
L
I
A
L
M
A
Y
I
G
S
Y
V
P
A
Q
K
V
E
I
G
P
I
D
R
I
F
T
R
V
G
A
A
D
D
L
A
S
G
R
S
T
F
M
V
E
M
T
E
T
A
N
I
L
H
N
A
T
E
Y
S
L
V
L
M
D
E
I
G
R
G
T
S
T
Y
D
G
L
S
L
A
W
A
C
A
E
N
L
A
N
K
I
K
A
L
T
L
F
A
T
H
Y
F
E
L
T
Q
L
P
E
K
M
E
G
V
A
N
V
H
L
D
A
L
E
H
G
D
T
I
A
F
M
H
S
V
Q
D
G
A
A
S
K
S
Y
G
L
A
V
A
A
L
A
G
V
P
K
E
V
I
K
R
A
R
Q
K
L
R
E
L
E
S
----------------------------------------------------------------------------------------