Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRLLPIKKLLGSTAGFRLASTKSSGTERLERLVEEVNGIKSKLKVLEESKAANGLVDLYASRKMKRLDIKRILSIFNDRAYHAP--IFCHKALPIILAHFITGLDKLPSGLNAMPSILAVRATLLRSFQKLINCKIPATDDQ--VQHFRRVLEDIDEEHAERDLLQTMAFGILELKEYVSSHRRALVDLKKTSERWASIPMTEENVLTYAEIQDIQAPLDSVNRCMITYNFISRMFLNHDPDMTMGSNPRRIGMVDLEMNLEHVVRNAVDEAKQICTDHYGDCPDTEFE---FTSDSKAFRFPYMSTTIRYIILELMKNAFRATVDSHMKRNDVGMVTCADMPPVRVLINLQEGTEHACICISDEGMGMTDEALAMAMAYSYTSVSKPALQLGDSGEGCASTAPSPLAGYGYGLPMSRVYAQSLGGDLFLQTMEGYGTRAYYYIKIADAQPLCDEETK 2Q8G Chain:A ((12-364)) ---------------------------------------------------GVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRH--NDVIPTMAQGVIEYKESF--------D-----------PVTSQNV---------QYFLDRFYMSRISI----RMLLNQHSLLFG----KHIGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHANR--------GVYPPIQVHVTL--GNEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVE---------TSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIK-------------

General information: TITO was launched using: RESULT: Template: 2Q8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1477 -44364 -30.04 -134.03 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -30.04 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_2Q8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q8G-query.scw PDB file : Tito_Scwrl_2Q8G.pdb: