Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRPCLLLLGIDVVFAHISGSCINHVGRADLVMLRRGFCRSIPLLGPTFTELVERLDSFEKEFIEWKAFRKAVESTSPKVTEAAKEIIAESMKEVKTSKVNLENPLTPEEFTELNQFYAKQKVKDIFFENLLYINTPNDLMQHSETVFRQYL-VRIARRVRHLNHAPYGLSQMPGIQMLKKWYQWSFHDVRSTPVPTTRD--ECYRCDRMVRRVFLRHYNVSSLITDGMVEFASREGWTHVDEEVMRTYDELQNFFEAFCLGRVRLRFLVGNYMYLSTKILGVSKEEYAVNDPDGLTVPIFFDHNAEDFVGQICKKCSLLVLTKCAIKEAQASYD------AEIEL-----KLAGDPNLVFVGIPYITYDIICAMLEDAVSANVDRQERTG-KACTKIEVTLAQWPTNKRFVLRISDTAGGMTLRQASKQLSCWSLYRNIQGHNQDTISTWTSSPIR-----LPYAYNAARVIGGNITLASIEGYGTDRQLYLPSTGLAGVSL 2Q8G Chain:A ((19-363)) --------------------------------------------------------------------------------------------------------------------FYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESF--DPV------TSQNVQYFLDRFYMSRISIRMLLNQHSLLFG------------------------KH-----IGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQP-IQVVYVPSHLYHMVFELFKNAMRATMEHHANRGVYPPIQVHVTLG----NEDLTVKMSDRGGGVPLRKIDRLFNY--MYSTAP---RPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYI----------

General information: TITO was launched using: RESULT: Template: 2Q8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1326 -50908 -38.39 -164.22 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -38.39 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.419

(partial model without unconserved sides chains): PDB file : Tito_2Q8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q8G-query.scw PDB file : Tito_Scwrl_2Q8G.pdb: