Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLRSAMHASDVDDAGRALQSTQRSIVSISRLTNEMSEVRAMSEGSFG-VVKCYRHDFDKLEYAVKQTKRPICGESNLQQQLQEIYALSSF-PHRHIVRYFDGWVEDRAVFVRLEKLDDCMASLPPPVSESV----------LTAMLHQTSTALYELHSHDVVHMDVKPENIL--RRQLDADTF--IFKLCDFGLARPLNGKDSVTGEHFLGLNDDDGDRRYMSPELLKNLHDVVGPPADMYALGKSCEAMMTATEDPSNTSARHL----------ESYSPAFIALIESMLCEDPARRPSAFQVVQATLPERLLSDGGLLELQRRIDAIRCEISDLDASDNDVMSSPRT 1X8B Chain:A ((7-282)) -----------------------------SRYTTEFHELEKIGSGEFGSVFKCVKR-LDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNG--GSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFIS---------KVMFKIGDLGHVTRISSPQV-----------EEGDSRFLANEVLQENYTHL-PKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSV----------------------------------------

General information: TITO was launched using: RESULT: Template: 1X8B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1089 34320 31.52 149.22 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 31.52 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_1X8B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1X8B-query.scw PDB file : Tito_Scwrl_1X8B.pdb: