Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPPKLKPLPAKKSTLPPELPEESDELTVGNIRVGPRGVEFTGEGDLQVRRPDLNFENLKRVRQLGQGTQGNVSMYVTADKSVFAVKKITIPVTLDTRTRQTVAAELRNIFSAQSNEYTVNLYNAFYRNRALRLVMEYMDWGNIDELIAEKVKIPPTVCGYIASQMLHALAVLHTKSNIVTEPNQHKSLRQIHRDIKPANVLLSTTGCVKLADFGIATSAETIGVNSFVGTATYMSPERIQGRRYSTPSDIWSVGVVVAQMLLGHYPFSSVSKGFMALLREINSV--EKYPLMDETGCSQETQDFIDSCLRQDPDDRSSAFELLESPWIKYCEEHGKAEMVALLASLGDRNRQYREDDALQAASRETSSLSLPRDTSETCKADNPLGM 3SLS Chain:A ((21-283)) ---------------------------------------------------ELKDDDFEKISELGAGN-GGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNS-PYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKI------------MHRDVKPSNILVNSRGEIKLCDFGVSGQLI------FVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIG-------AIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIK----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3SLS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1210 -102916 -85.05 -413.32 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -85.05 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_3SLS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SLS-query.scw PDB file : Tito_Scwrl_3SLS.pdb: