Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDSKQCEAFLAVAEVGSFDAAGEYLCITPSAVSLRVQALEKYLGQILIIRGRPCVLTQAGQTLLQHLRHTRLMEQNLLQGLMGKSSDSEFYKIALASNADSLATWLLPSIQQTLFK--EKIVLELKIDDQSHTHTLLETGQVNACITAEEQVMAGCLAQPLGKMRYKMLASAEFVNRWFSGGINREVLRKTPAVIFNHKDLMHSEVLLKGYGLPMQSYPYSFIPATDAFVKAIQLGLGYGMVPELQVQPLLENETLVDIMPEAQLDVPLYWHHWKRQSKQLDVLTETIVESAKQILR 3ISP Chain:A ((6-292)) -LDGPQLAALAAVVELGSFDAAAERLHVTPSAVSQRIKSLEQQVGQVLVVREKPCRATTAGIPLLRLAAQTALLESEAL-AEMG--ASLKRTRITIAVNADSMATWF-----SAVFDGLGDVLLDVRIEDQDHSARLLREGVAMGAVTTERNPVPGCRVHPLGEMRYLPVASRPFVQRHLSDGFTAAAAAKAPSLAWNRDDGLQDMLVRKAFRRAI-TRPTHFVPTTEGFTAAARAGLGWGMFPEKLAASPLADGSFVRVC-DIHLDVPLYWQCWKLDSPIIARITDTVRAAASGL--

General information: TITO was launched using: RESULT: Template: 3ISP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1386 -42974 -31.01 -151.85 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -31.01 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_3ISP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ISP-query.scw PDB file : Tito_Scwrl_3ISP.pdb: