Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKPPYHDDQASFSAPIEDLQVRIAFLDDLVEELNQQLAIQTLEIADLKKQMQLLYQRVESA-----DLSEGVAPFDPLTNKPPHY 5NNV Chain:A ((26-123)) --------------AKIEDTRDKIQALDESVDELQQVLLVTSEELEKLEGRKEVLKERKKNAVQNQEQLEEAIVQF----------

General information: TITO was launched using: RESULT: Template: 5NNV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 9 -608 -67.56 -10.67 target 2D structure prediction score : 0.86 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -67.56 2D Compatibility (Sec. Struct. Predict.) : 0.86 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_5NNV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5NNV-query.scw PDB file : Tito_Scwrl_5NNV.pdb: