Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPKSFLIYKNDKLDLCKTLAIIEERLELPSPMEQDDMAGKTLYDKLWDDHVVTQRDDGSCLLYIDRHLLHEVTSPQAFEGLQLAG-RQPWRLSANVATPDHNVPTSKKERDQGIAGIEDDTSRIQVQTLDDNCKAFNIVEFGIN---DIRQGIVHVVGPEQGLTLPGMTVVCGDSHTATHGAFGCLAHGIGTSEVEHVLATQCLVQKKSKNMLVRVDGVLGKGVTPKDVVLAIIGKIGTAGGTGYAIEFGGQVFRDMSIEGRMTVCNMAIEAGARVGMVAVDDKTIEYVKGRSYAPKGEQWDQAVAYWNTLHSDDDAVFDAVVELNGAEIEPQVSWGTSPEMVIPVSKAVPTLEQAKDDVQRNDWTRAYQYMGLNAGQALADIQLGRVFIGSCTNSRIEDIRAAAEVVKGRKVAPSIKQAMIVPGSGLVKQQAEKEGLDKIFLEAGFEWREPGCSMCLAMNADKLQPGEHCASTSNRNFEGRQGN-GGRTHLVSPAMAVAAAIAGHFVDIRSF 4NQY Chain:A ((48-442)) ----------------------------------------------------------------IDVAMVHDITGPLTVNTLKEYGIEKVWNPEKIVILFD---------------------NHILMRKF--------VKEQGIKYFYDIREGVCHQVLPEKGHVAPGEVVVGADSHTCTHGAFGAFATGIGSTDMAHVFATGKLWFKVPETIYFNITGDLQPYVTSKDVILSIIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYVK-EAMKKHGTERP-----FEVIKGDEDAEFAEVYEIEADKIEP----------VFACPHNVDNVKQARE---------------------VAGKPIDQVFIGSCTNGRLEDLRMAIKIIEKHGGIADDVRVVVTPASREEYLKALKEGIIEKFLKYGCVVTNPSCSACMGSLYGVLGPGEVCVSTSNRNFRGRQGSLEAEIYLASPITAAACAVKGELVDPRD-

General information: TITO was launched using: RESULT: Template: 4NQY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2265 -4209 -1.86 -11.13 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -1.86 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_4NQY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NQY-query.scw PDB file : Tito_Scwrl_4NQY.pdb: