Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLALFDLDHTLLNTDSDHSWGEFLVNEGLV-DPVHHRQMNDKFYEDYKAGQLDPYAYNEFVFGFLTKHDNNYLTELHQLFMEKVIRPKMRPKGFDAIKKHQDLGHTIVGITATSDFITAPIFREFGITEILATNAEVADGKYTGKVAGLACYQKGKLARLEAWLDGRSV-----SESWAYSDSINDRFLLEYATHAIAVNPDDRLEKLAQEHNWEIQDWSI 3FVV Chain:A ((5-222)) -RLALFDLDHTLLPLDSDYQWADFLARTGRAGDPAEARRRNDDLMERYNRGELTAEQAAEFMLGLLAAHSPVELAAWHEEFMRDVIRPSLTVQAVDVVRGHLAAGDLCALVTATNSFVTAPIARAFGVQHLIATDPEYRDGRYTGRIEGTPSFREGKVVRVNQWLAGMGLALGDFAESYFYSDSVNDVPLLEAVTRPIAANPSPGLREIAQARGWQVID---

General information: TITO was launched using: RESULT: Template: 3FVV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1022 -15336 -15.01 -72.34 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -15.01 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_3FVV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FVV-query.scw PDB file : Tito_Scwrl_3FVV.pdb: