Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQASPVYQGSCLCQGIRYEIK--GDIGDIIQCHCQRCRKSNGTAFATNAPINSADFKITQG-EDL-IKKFAVNGVYRWFCSECGSPLISSRDAQPELYRLRIGTLDTPLDQKPTMHIFAASKAEWECIHDDLPQYDERP 3FAC Chain:A ((2-96)) --------KGTCHCGAVEIEVELLNGFADARRCDCSFCRRRGAIA----ATARLSDLRVVRGAENLTLYQFGTRTAKHWFCRTCGIYTHHQRRSNPEEYGVNVAILE---------------------------------

General information: TITO was launched using: RESULT: Template: 3FAC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 398 2090 5.25 22.96 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 5.25 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.247

(partial model without unconserved sides chains): PDB file : Tito_3FAC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FAC-query.scw PDB file : Tito_Scwrl_3FAC.pdb: