Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRAYFEKLLDSSQQQKQLPLIFPLLIALFSGAVFSFALAPYYWWWLAILSPALLYATLHNRSAKQAFAIGWSYGFGLWFVGAFWLYTSIHVYGDTNAFLSVCMIAVMALVMGLFTAFQTWVYRRFFPET-----PLTFAPLWIIFEWAKTWVFTGFPWLFVGYAFTERLLDGYAPLFGIYAISFVVIVLACALVEVLRKRIFWVIPAALLVLG-AWGASYIQFVQPKATKPLSVSLIQGNIPQDLKWLTEYQVRTLEIYAGLTQSEWGRD-LIVWPESSIPLFQTDIEPFLDAMDAQAKKNHTAWVTGIPYWDVTKSHQVGSPLYYNSIMASGS------DSSGLYKKQRLVPFGEYIPLSGLLSWVLPAMQNDISMSGFTRGESDQKPLLIKGHALAAAICYEVAYPNLTRRN-AEDSDFLVTVSNDAWFTGTAGPWQHLQMVQMRAKENGRWFIRATNTGVTAFIDQNGHITEQAPIDKEFVLRGDLPAMQGQTFYNRLGDYP------ILGFAVLLLVLGWIYRPRKVDVSYKSRR 5N6H Chain:A ((34-527)) -----------------------LLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPLKGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQPEKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDT---YNTIITLGKGAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFF--DLPMSSFSRGPYIQPPLSANGIELTAAICYEIILGEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSL-----------------

General information: TITO was launched using: RESULT: Template: 5N6H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2691 -135268 -50.27 -285.37 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -50.27 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.419

(partial model without unconserved sides chains): PDB file : Tito_5N6H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5N6H-query.scw PDB file : Tito_Scwrl_5N6H.pdb: