Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEVLHHDNHGHDGHHEHDDTDLTVFGFWTYLMSDLILFGSLFIAFAVLSSHVPPGTPSAYDLFHESLGYVLTETFALLISSVTFGFAVLASYKKNVNQVITWLFITFLFGATFIGMELYEFHHLVEEGHGPTHSAFLSSFFTLVGTHGIHVTSGLVWMLVLMYQIKTKGLTLPNTRRLACLSLFWHFLDIVWICVFSVVYLLGVL 1FFT Chain:C ((19-203)) -----------------HDAGGTKIFGFWIYLMSDCILFSILFATYAVLVNGTAGG-PTGKDIF--ELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWLALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVLMVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGA-

General information: TITO was launched using: RESULT: Template: 1FFT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 608 -134127 -220.60 -725.01 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.88 3D Compatibility (PKB) : -220.60 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.336

(partial model without unconserved sides chains): PDB file : Tito_1FFT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FFT-query.scw PDB file : Tito_Scwrl_1FFT.pdb: