Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNISYELEKKIVNLNALYNEEEAELSLRLSQNIRKNQQDGYVYSLISRGFEIKGLKLWLVDKNIDQLKQWFYVSSVLRAESCKYSSGYTLSTPDEFIFPLLSDNTEIINKFAYLDTVNLLWGEFEPSYQEAKFKP-SKDDPRFRTHALQAVLRNDWEDYKKIKEVANQKINKLREVVQFEFGIYDAIYEKDKDKIIEIVQTLLNPRVHKTYNKDFGEEFNGEIWSHHPVMFTKLAWMNGLEIEIDNPLVPMELMPIKPLEHYDYHYDFLDPNWKPKSLFEKLFGKKRNK 5FXD Chain:A ((362-486)) ---------------------------------------------------------------------------------------------------VLQDRHKINNGIPSLDELQLL--DWVPNGGHIGFSPVSAPDGREAMKQFEMV-RNRANEYNK--DYAAQFIIGLREMHHVCLFIYDTAIPEAREEILQMTKVLVREAAEAGY---------GEYRTHNALMDDVMATFN---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 466 -4552 -9.77 -36.71 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -9.77 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.315

(partial model without unconserved sides chains): PDB file : Tito_5FXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FXD-query.scw PDB file : Tito_Scwrl_5FXD.pdb: