Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLNSSIDKKMTTLNILFKTTSLCLAMALLVGCNDNDDNDSSTPAPRSNQTVNILAFNDFHGNLEPPKRYVEAANPNDASQTVRIPVGGVSYFADAIKKLKAQNPNNVVVSAGDLISASPLTSSLFLDEPTIEVMNDIQIDFNAVGNHEFDRGTDELRRLQNGGCQQYTSTAPCQINKNFSGAKFNFLAANV-AMKNDTSKTIFPAYKVKTYGGIPVAFIGLTLKATPSIVSAAGIKDVEFRDEADTVNALIPELQKQGIEAIVVVVHEGAAPSTKLNQKTCDGLSGPILGILDRLNPAVDIVVSGHTHQSYICDYATK--NPAKPF------------LLTSAGQYGTLITDIKVGLDGKTGDIIKKDAKQIPVQSEAYTSGTTTVSLTDLYQKFSKTPSIEAILDKYRQAVTTISGRVVGTSTA--VVSRTQVESGESPLGDMIADAQQAAALQASNQGSDFTLMNPGGVRADLLINSSNQITFGDIFAVQPFGNSIVTLSLTGKQIRDVLEQQWSGANANSPRILQPSKELSYQYAANTSVSPRASNIMVAGS----PLVDTKVYRVTVNSFLADGGDNFTVLKEGQN-RVGGG-QDIDALESYVAQNSKDSPLIIPATTRIKVIK 2Z1A Chain:A ((29-516)) --------------------------------------------------TLTLVHTNDTHAHLEP----VELTLSGE-----KTPVGGVARRVALFDRVWARAKNPLFLDAGDVFQGT-LYFNQYRGLADRYFMHRLRYRAMALGNHEFDLG-------------------PGPLADFLKGARFKVVSANVDASREPRLKGLFAPYAVVVVGGERVGIIGLTTPDTREISNPG--PTVAFLDPYESAQKAVYELLAKGVNKIVVLSHLGYGEDLKLARRLV----------------GVQVIVGGHSH-TLLGSFPHKELSPAGPYPTVVKNPEGKDVLVVQAWEWGKVVGLLEVTFDAK-GELLAYKGEALLMTPEA--------APEDFFAK-------EALL-AYAQPVMALMQQVIAEAKVDLVGERAVVRRRESNLGNLITDGM---LWKTRNAGTQIALQNGGGIRASI---PKGPITVGKVYEVLPFGNTLVVMDLKGKEILAALENGVSQWENTAGRFLQVSG-LRYAFDLSRPAGSRVVRVEVKTEKGYVPLDLEATYRVVVNNFIANGGDGFTVLKEAQGYRVDTGFSDAESFMDYLKE-------------------

General information: TITO was launched using: RESULT: Template: 2Z1A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2867 -45154 -15.75 -97.11 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -15.75 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_2Z1A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z1A-query.scw PDB file : Tito_Scwrl_2Z1A.pdb: