Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNALTQELVELLTLEKLEENIYRGISRNLVGKRVFGGQVLGQALRAASYTT--DRPAHSLHAYFLYGGDINAPIIYEVDRLRDGKSFVSRQVRAIQHGRVIFSAMVSFANPEEGLNYQHPEPDYPAPEALKSESELKEGILNFVPENVRASFMRERHVEIRPIDPVNPFQPQPEAPFNAHYIRTHDRIPKQLEDISLHQAIVAFYSDFTLMTTALRPHGLSYISPSLQCASIDHAIYFHRPLRADEWMLYDMEATVSAASRGLNFGRMW-QNGQLVCSTVQEGLMRLREIETQ 4QFW Chain:A ((17-284)) ----------------IEEGIFRGQSE-----QVFGGQVVGQAIYAAKQTVPAERTVHSFHSYFLRPGDSSKPIIYDVETLRDGNSFSARRVSAIQNGKPIFYMTASFQSQEEGFEHQNTMPDVPPPEGLMSETDIARQ--------------GPQPIEMRPVKFHNPLQGSVEEPNRYVWFRANGKMP---DDLRVHQYLLGYASDFNFLPTALQPHGIGFLEPGMQIATIDHSMWFHRPFRLDDWLLYAVESTSASGARGFVRGQIYNREGVLVASTVQEGVIRL------

General information: TITO was launched using: RESULT: Template: 4QFW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1272 -31514 -24.77 -128.10 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -24.77 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4QFW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QFW-query.scw PDB file : Tito_Scwrl_4QFW.pdb: