Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMQDTVADMLTRVRNAQMAKKQTVSMPSSKLKVAIANVLQQEGYISNVEVAQEETKSTLTITLKYFEGKPVIEMVKRVSRPGLRQYRGKDKLPSVKQGLGIAIVSTSKGIMTDRAARAAGIGGEVIAFVS 5ME0 Chain:H ((2-130)) -SMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEGFIEDFKV-EGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIAVVSTSKGVMTDRAARQAGLGGEIICYVA

General information: TITO was launched using: RESULT: Template: 5ME0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 596 -72761 -122.08 -564.04 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain H : 0.93 3D Compatibility (PKB) : -122.08 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_5ME0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ME0-query.scw PDB file : Tito_Scwrl_5ME0.pdb: