Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKDIKVDPAQLEAALDARLKIRAGFDKPTAGMAAGMTQVNMISVPRDWAYDFLLYAHRNPQSCPVLDVLEEGIYATKLAADSDIRTDFPRYRIWKDGEMVDEVTDAREIYNAHPDLVTFLIGCSFSFETALQEAGIEVRHIHDDTNVPMYLSNIKCEPAGRISGNMVVSMRPIPSHQISEAVKITARMPSVHGAPVHIGHPESLGIKDVNKPDFGDASRIEAGEIPVFWACGVTPQAAVINSKIPFAISHAPGYMFITDIPDRAWMG 3DB9 Chain:A ((14-266)) ------------EAARKARATYRDGLVAPTSGIAPGFTQANMIVLPRDWAFDFLLYAQRNPKPCPVLDVSDPGSPTTLLAPGADLRTDLPLYRIWRDGKLAEETADATSAWAERDDLVAFLIG------TPMVEAGI--------SNVPMYLTNRPCRPAGRLKGNMVVSMRPIPASRVADAATISGR-------PVHVGAPEQIGISDLSKPDFGDAVRIEPGEVPVFWACGVTPQAAVMASGVPFAITHAPGHMFITDIPDTA---

General information: TITO was launched using: RESULT: Template: 3DB9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1205 -140102 -116.27 -603.89 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -116.27 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_3DB9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DB9-query.scw PDB file : Tito_Scwrl_3DB9.pdb: