Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPNLVVFSGNAHPQFAQKVVSHLHIPLGAASVGQFSDGEISVEITENVRGKDVFIVQPTCAPTNDNLMEILVMADALRRASAGRITAVIPYFGYARQDRRPRSARVPITAKVVADMLTTVGIDRVVMIDLHADQIQGFFDIPVDNIYGTPALLADLRQQ--QHDNLMVVSPDVGGVVRARAVAKQMGDIDLAIIDKRRQKANESQVMHLIGDVKDRDCVIVDDMVDTAGTLCKAADALKQFGARRVVAYATHPVLSGKAIENLRNSVIDELVVTDTIPLSEEALNLGKIRQVSVASMVAETIRRINNEESISAMFDSL 4LYG Chain:B ((3-315)) --NIKIFSGSSHQDLSQKIADRLGLELGKVVTKKFSNQETCVTIGESVRGEDVYIVQSGCGEINDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKD-KSRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIPVDNLYAEPAVLKWIRENISEWRNCTIVSPDAGGAKRVTSIADRLN-VDFALIHKERK-----DRMVLVGDVKDRVAILVDDMADTCGTICHAADKLLSAGATRVYAILTHGIFSGPAISRINNACFEAVVVTNTIPQEDKMKHCSKIQVIDISMILAEAIRRTHNGESVSYLFSH-

General information: TITO was launched using: RESULT: Template: 4LYG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1734 -108758 -62.72 -355.42 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -62.72 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.604

(partial model without unconserved sides chains): PDB file : Tito_4LYG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LYG-query.scw PDB file : Tito_Scwrl_4LYG.pdb: