Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGMEEHLLTQKLQQRLRFSTHIQEAQAAVLIAITNENNPKVLLTRRSIHMNNHAGEVSFPGGKRDPSDTSNIVVALREAQEETALNPFDVQLLGDLPMQRARSGLSVKPIVGLIP----------PEVTLIPQPTEIDRIFFVPLQQLIEMRPTPYEVRYAHQSLY-FPSLQIDNEIIWGLTAQMLIALFKYGLGYQKEWPFLLNAPTFGMPKFSHKK 1NQY Chain:A ((35-190)) --------------------------RAAVLVALTREADPRVLLTVRS------KGQIAFPGGSLDAGETPT-QAALREAQEEVALDPAAVTLLGELDDVFTPVGFHVTPVLGRIAPEALDTLRVTPEVAQIITPT-LAELRAVPLVRERRTLPDGTEV-----PLYRYPWRGLD---IWGMTARVLHDLLE---------------------------

General information: TITO was launched using: RESULT: Template: 1NQY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 607 -45651 -75.21 -328.42 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -75.21 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_1NQY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NQY-query.scw PDB file : Tito_Scwrl_1NQY.pdb: