Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTISKGVSLMKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEATGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYEIPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF 2X8W Chain:A ((4-122)) ------------LALIAHDAKKEEMVAFCQRHREVLARFPLVATGTTGRRIEEATGLTVEKLLSGPLGGDQQMGARVAEGRILAVIFFRDPLTAQPHEPDVQALLRVCDVHGVPLATNPMAAEALIPWLQS-------------------

General information: TITO was launched using: RESULT: Template: 2X8W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 575 -91847 -159.73 -771.82 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -159.73 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_2X8W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X8W-query.scw PDB file : Tito_Scwrl_2X8W.pdb: