Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNIQDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIGAGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQR 3VNP Chain:A ((18-171)) ---FIADYVTITGDVVIGEETSIWFNTVIRGDVAPTVIGNRVNIQDNSILHQSPNNPLIIEDGVTVGHQVILHSAIVRKNALIGMGSIILDRAEIGEGAFIGAGSLVPPGKKIPPNTLALGRPAKVVRELTEDDIREMERIRREYVEKGQYYK--ALQQ--

General information: TITO was launched using: RESULT: Template: 3VNP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 873 -114431 -131.08 -743.06 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -131.08 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_3VNP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VNP-query.scw PDB file : Tito_Scwrl_3VNP.pdb: