Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKILVFGHQNPDTDAIGAAIAFSHLQNE-LGKETEAVALGTPNEETQYALDHFQLSAPRVVESAKAENAD-VMLVDHNEFQQSISDISEVNILAVVDHHRIANFETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTPEDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQVNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEEAFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS 1WPP Chain:A ((2-310)) -SKILVFGHQNPDSDAIGSSYAFAYLAREAYGLDTEAVALGEPNEETAFVLDYFGVAAPRVITSAKAEGAEQVILTDHNEFQQSVADIAEVEVYGVVDHHRVANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTTHPTDKVIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRVAQVNTVDIAEVLERQAEIEAAIEKAIADNGYSDFVLMITDIINSNSEILAIGSNMDKVEAAFNFVLENNHAFLAGAVSRKKQVVPQLTESFN

General information: TITO was launched using: RESULT: Template: 1WPP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1807 31628 17.50 103.02 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.91 3D Compatibility (PKB) : 17.50 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_1WPP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WPP-query.scw PDB file : Tito_Scwrl_1WPP.pdb: