Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYAGPMERAGAESILANRSDGTFLVRQRVKDAAEFAISIKYNVEVKHIKIMTAEGLYRITEKKAFRGLTELVEFY 2LCT Chain:A ((11-85)) WYAGPMERAGAESILANRSDGTFLVRQRVKDAAEFAISIKYNVEVKHIKIMTAEGLYRITEKKAFRGLTELVEFY

General information: TITO was launched using: RESULT: Template: 2LCT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 335 -45635 -136.22 -608.47 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -136.22 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_2LCT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LCT-query.scw PDB file : Tito_Scwrl_2LCT.pdb: