Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVCSILASTSVLAVWVDGGQWNYGVGWSGNFGYSDYLHSTRSHTATVKDGN--KFSKDRAEAEAWARASIFKFPPTGMEYFYGF 2LGN Chain:A ((2-65)) ------------------GTWQHGYGVSS--AYSNYHHGSKTHSATVVNNNTGRQGKDTQRAGVWAKATVGRNLTEKASFYYNF

General information: TITO was launched using: RESULT: Template: 2LGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 311 4530 14.57 73.06 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 14.57 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.064

(partial model without unconserved sides chains): PDB file : Tito_2LGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LGN-query.scw PDB file : Tito_Scwrl_2LGN.pdb: