Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWVKDMFILGSGDQGLAVLSIYEGEVEGLVDIFEEGRFIGCKILDKTVLGGLKWFESLLLTLDRK---PRAIVAFGDNFKREEIFCSYKNK-VEYINVICNSARIFKHSFLGKGNFIGTNVTIQALVEIGDNNIINSGSIVSCNCKIGNNVNISPGVILSGNVKIDDNVFIGAGATIRDAVSIGFGAIIGAGATVIHNVPENAVVVGTPGKIIKYRSV 4EAB Chain:A ((49-218)) ---------------------------------------------RAVLGVPVVGDDLALPMLREQGLSRLFVAIGDNRLRQKLGRKARDHGFSLVNAIHPSAVVSPSVRLGEGVAVMAGVAINADSWIGDLAIINTGAVVDADCRLGAACHLGPASALAGGVSVGERAFLGVGARVIPGVTIGADTIVGAGGVVVRDLPDSVLAIGVPAKIKGD---

General information: TITO was launched using: RESULT: Template: 4EAB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 916 -32501 -35.48 -195.79 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -35.48 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_4EAB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EAB-query.scw PDB file : Tito_Scwrl_4EAB.pdb: