Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITFYLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQGDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCILMLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDLGRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEGFTFEKE 3HLU Chain:A ((25-75)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELDLGVTMLQAVGAYKNNETEVIMCVMRKATLVKVRNLLKEVDPDAFMIVS---------------

General information: TITO was launched using: RESULT: Template: 3HLU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 85 -21306 -250.66 -417.76 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.44 3D Compatibility (PKB) : -250.66 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.44 QMean score : 0.440

(partial model without unconserved sides chains): PDB file : Tito_3HLU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HLU-query.scw PDB file : Tito_Scwrl_3HLU.pdb: