Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGTDHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE 5HUH Chain:A ((34-307)) MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYVLGISGGQDSTLAGKLAQMAIAELREETSDQAYQFIAVRLPYGVQADEADAQKALAFIAPDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGTDHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYE-------------LADEVALGVTYQDIDDYLEGKLISKVAQATIEKWWHKGQHKRHLPITIFADFWK

General information: TITO was launched using: RESULT: Template: 5HUH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1221 -91362 -74.83 -350.04 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.93 3D Compatibility (PKB) : -74.83 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_5HUH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HUH-query.scw PDB file : Tito_Scwrl_5HUH.pdb: