Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMSHLRFVVHIARSYAGYGLPQGDLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLE-EAQSIANDLNVTPEQV---LEMEGRLTAYDAAFEAQGDDDDDTPHTAPVLYLEDNRYDPARLVENEDWEEQSTSALHDAMNQLDDRSRNILQRRW--LDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS 5IPL Chain:F ((61-314)) ----------------------YLGEIGYSPLLTAEEEVYFARRALRG-DVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIV-KELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGG------DSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLF----ELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLR------

General information: TITO was launched using: RESULT: Template: 5IPL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 744 -35384 -47.56 -142.68 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain F : 0.77 3D Compatibility (PKB) : -47.56 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_5IPL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IPL-query.scw PDB file : Tito_Scwrl_5IPL.pdb: