Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTANIMTTLKNQITDALKTSMRAKDMATVTVLRSLQAAIKQIEVD-ERIELDDAQVLAVIEKQIKQRKESIKAFEGAGRDDLASKEQAEAEVLSQFLPEAMTEEELDSLIEQTIAAQEATSMKDMGKVMNSLRPIIAGRADPAQVSAKIKAKLA 1NG6 Chain:A ((2-148)) -------SLLERLNQDMKLYMKNREKDKLTVVRMVKASLQNEAIKLKKDSLTEDEELTVLSRELKQRKDSLQEFSNANRLDLVDKVQKELDILEVYLPEQLSEEELRTIVNETIAEVGASSKADMGKVMGAIMPKVKGKADGSLINKLVSSQLS

General information: TITO was launched using: RESULT: Template: 1NG6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 468 12003 25.65 82.21 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 25.65 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.711

(partial model without unconserved sides chains): PDB file : Tito_1NG6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NG6-query.scw PDB file : Tito_Scwrl_1NG6.pdb: