Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKFVDDHHLKRKVGTLEMVVAMLLSGSIGLFVIKSGQSPINIVFFRCLISALCLIPICYFYGHFRKVYFGKKELFLMVTSGLLIIFNWVLLFAAFPKTSISLATIVYHVNPFVILFLGALVFHEKLNKNDVLWTIIAFIGLIVIIGLGSASVN-SNELVGLGLVLIATT-LYSISVLITKKLSNTPPLLIVFIQTLSGAIVMAPFISVFENP--PIGQQWLFVVGLGVLHTAFLYYLMYSAIKKIPLNNIAILSFIYPISTIVIDYFFFDHVLTSTQVLGAGLILLGVLGVKLHWNIFAVKKVGIRN 5I20 Chain:A ((83-249)) --------------------------------------------------------------------------------------------FSALKLAPPAEAGLVAYLWPLLIVLFSAFLPGERLRPAHVAGALMGLAGTVVLLGARAGGFGFAPEYVPGYLAAAACAVIWSVYSVASRRFARVPTEVVAGFCLATAALSALCHI-LFEPSVWPVGSEWLAVVALGIGPVGIAFYTWDIGMKRGDVRLLGVLSYAAPV------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5I20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 492 -73392 -149.17 -450.26 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -149.17 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.189

(partial model without unconserved sides chains): PDB file : Tito_5I20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I20-query.scw PDB file : Tito_Scwrl_5I20.pdb: