Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQQVIIVGGGMVGLSLSLMLAKANIAVKLLEA-VKYPNYDDQNVAPYHSSFDARNTALSRRSVQIYQKLGLW-DALQQHATPILQVHITEQGSFGKARLIAEQEKVESFGQVIENAWLGRVLLTQVRQQPLIELIDGVQVTALTQDAEQVHIEAQRGDEVLKLESKLLIAADGRDSFCRQAIGVGVDVHDYDQVAIVTTVQTSKPHEHVGFERFSALGPLALLPLPGEYRRSVVWPVKKGTEGEWLGEENDQHFLDALQKTYGDRAGKFEKTGKRFSYPLSQVLAHKQAVGRIILMGNAAHTIHPVAGQGFNLCLRDADVLLRYLVNQLSASDDIGNPDNLLAYEEARLSDQQRVIKFCDTVVRGFSNQNPLLKLIRNTGLIAFDVIPGVKPLVANYAMGLKA 4K22 Chain:A ((6-363)) ----VAIVGGGMVGLAVACGLQGSGLRVAVLEQR-----------PP-----QLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYSHLGHIVENSVIHYALWNKAHQSSDITLLAPAELQQVAWGENETFLTLKDGS---MLTARLVIGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLSDPHLCSIVWSLSP-EEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYARQFASHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRRYERSRKHSAALMLAGMQGFRDLFSGTNPA-------------------------------

General information: TITO was launched using: RESULT: Template: 4K22.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2028 -42048 -20.73 -121.17 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -20.73 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_4K22.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K22-query.scw PDB file : Tito_Scwrl_4K22.pdb: