Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVVAQQLGQWAEQTALKLLKEQNYEWVASNYHSRRGEVDLIVKRGNELIFVEVKARGQGNYGQACEMVTLSKQKKIIKTAMRFLQRYPSYQDFYCRFDVICFDFPQKIAKTVQQDFSKFHYDLQWIENAFTLD 3FOV Chain:A ((25-113)) ---------SAEASAADYLERQGYRILARRFKTRCGEIDLVAQRDALVAFVEVKAR--------AYAVTPRQQSRIVAAAEAWLSRHPEHAMSELRFDAI---------------------------------

General information: TITO was launched using: RESULT: Template: 3FOV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 322 -42802 -132.92 -515.68 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -132.92 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.695

(partial model without unconserved sides chains): PDB file : Tito_3FOV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FOV-query.scw PDB file : Tito_Scwrl_3FOV.pdb: