Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGSTITPWVVGNWKMNPMRANANQLIEEFKQLLQQNQIADENCHVGVAPVSIALTTVQAQLQDAARTVYTVAQDVSRVAGTGAYTGEVSAELLKDSQINFVLVGHSERRDIFGDNVEILKAKLQNALNAGMTVIYCVGESLEQREQGQAEQVVLQQICDIAPVVTAEQWQNQVVIAYEPIWAIGTGKTASPQDAQAMHAKIREGLCQLTPAGSNIA----ILYGGSVKAENAVELAACPDINGALVGGASLNAASFYQIVQAFAQSK 3QST Chain:A ((6-255)) ------TFFVGGNWKANPKTV---QEAEKLVEMLNGAKV-EGNVEVVVAAPFVFLPTLQQKL----RKDWKVSAENVFTKPNGAFTGEVTVPMIKSFGIEWTILGHSERRDILKEDDEFLAAKAKFALENGMKIIYCCGEHLSEREAGKASEFVSAQIEKMIPAIPAGKW-DDVVIAYEPIWAIGTGKVASTQDAQEMCKVIRDILA--AKVGADIANKVRILYGGSVKPNNCNELAACPDVDGFLVGGASLEAG-FINIVNSNVHSK

General information: TITO was launched using: RESULT: Template: 3QST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1303 -118159 -90.68 -480.32 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -90.68 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_3QST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QST-query.scw PDB file : Tito_Scwrl_3QST.pdb: