Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKPDISELSVEELKRLQEEAEALIASKKDQAIEDAYNQIIEIAENVGFSVEQLLEFGAQKRKKTTRKSVEPRYRNKNNAEETWTGRGKQPRWLVAEIEKGAKLEDFLI 2LEV Chain:A ((18-51)) ------------------------------------------------------------------------YRNEEG--QTWSGVGRQPRWLKEALLNGMKKEDFLV

General information: TITO was launched using: RESULT: Template: 2LEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 89 1525 17.13 44.84 target 2D structure prediction score : 0.91 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 17.13 2D Compatibility (Sec. Struct. Predict.) : 0.91 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.915

(partial model without unconserved sides chains): PDB file : Tito_2LEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LEV-query.scw PDB file : Tito_Scwrl_2LEV.pdb: