Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVQIITIDGPSGSGKGTLAAKLAAYYQFHLLDSGALYRLLGLS-LHKH-DLLEKLDSHLDECVNYARQLNIKFETSAEGTLVFLDGEDVTQTIRTERVGEYASKVAAIPELRQALFERQRAFAQTPGLVADGRDMATSIFPEANAKIYLTASAESRAERRVKQLQGMGLDAKINDILANIQARDKRDMEREVAPLKPAGDAYIIDSSELTIDQVFKLMVDYVNSRTVSN 2CMK Chain:A ((7-223)) ----VITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLALAALHHHVDVASE-----DALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQVAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRMLQLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQYARQK----

General information: TITO was launched using: RESULT: Template: 2CMK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1003 -4024 -4.01 -18.72 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -4.01 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.632

(partial model without unconserved sides chains): PDB file : Tito_2CMK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CMK-query.scw PDB file : Tito_Scwrl_2CMK.pdb: