Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIEIRTDKNIHNSERLITYVRAELTQEFQRHSERITHFSV-HFSDENGDKGGDKDIHCMIEARPSGLKPVAVHHKAGNIDASIHGAIEKLKRSLEHTFEKKEHPRGGQPEFIDDEV 5THG Chain:A ((209-263)) -------DKNLMNS--LITYLGCGTIQEKNRSKGSMLHFIVTKFSDIN-----DKIIPVFQENTLIGVK------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5THG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 98 -7117 -72.62 -131.80 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -72.62 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.003

(partial model without unconserved sides chains): PDB file : Tito_5THG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5THG-query.scw PDB file : Tito_Scwrl_5THG.pdb: