Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHPYAPTLQRVLLFGLFFILIFLGFNILKYFIVPVLWAAIIAYMTWPIYLRIQHFFGENRNNLNATVMISLVILIVGVPFTCAIFILQHEGRNLYFDLQRQVFSGHLSVPDFIRNLPFIGKEISRTLNEINTDPNSTIQNIAAWVQSHLSYGRVLLNEISKNIVKLCFAILSLFFFYRDGHTILTQVSKALEMVIGPRIHHYLDTISETTRAVVYGVGLTAIAQAVLAGLSYFVAGVPNPMVLTIATFLLALIPFGTPVSYLGVGLWLFSQGQTMEAIGVILWGVLIVSSSDNVIRPLVISGATQIPFLLIMFGVLGGIASFGLVGLFIGPVILAVLLAIWREWLHETIDPEPMPKTTMIYDSDDDLPPSKN 4ZXA Chain:W ((120-150)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FETPLIMATFKAILRDWTNATFDPFAAPEET--------------

General information: TITO was launched using: RESULT: Template: 4ZXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain W - contact count / total energy / energy per contact / energy per residue : 21 -1761 -83.86 -56.81 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain W : 0.40 3D Compatibility (PKB) : -83.86 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.40 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_4ZXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZXA-query.scw PDB file : Tito_Scwrl_4ZXA.pdb: