Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDVQVHQLYIHGRYVEATSGKTFNSINPANGEIIATLQQASEQDIEAAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELARLETLDTGKAYSETSTVDIVTGADVLEYYAGLATAIQGEQVPLRESSFFYTRREPLGVVAGIGAWNYPIQIALWKSAPALAAGNAMIFKPSETTPLTALKLAEIYTEAGLPDGVFNVVQGAGREIGQWLTEHPVIEKISFTGGVETGKKVMASAAGSTLKEVTMELGGKSPLIICEDADLNRAADIAVMANFFSSGQVCTNGTRVFVPKSRLADFEKAVVERVKRILIGDPMAEDTNFGPLTSFPHMEKVLSFIESGKQQGAKVLIGGGRATEGELAKGAYVLPTVFSDCTDQMAIVQEEIFGPVMSILGYETEEEVIQRANDTTFGLAAGVVTQDISRAHRIIHQIEAGICWINTWGESPAEMPVGGYKQSGVGRENGLTTLGHYTRIKSIQVELGDYQGIF 2WME Chain:A ((2-490)) -ARFEEQKLYIGGRYVEASSGATFETINPANGEVLAKVQRASREDVERAVQSAVEGQKVWAAMTAMQRSRILRRAVDILRERNDELAALETLDTGKPLAETRSVDIVTGADVLEYYAGLVPAIEGEQIPLRETSFVYTRREPLGVVAGIGAWNYPVQIALWKSAPALAAGNAMIFKPSEVTPLTALKLAEIYTEAGVPDGVFNVLTGSGREVGQWLTEHPLIEKISFTGGTSTGKKVMASASSSSLKEVTMELGGKSPLIIFPDADLDRAADIAVMANFFSSGQVCTNGTRVFIHRSQQARFEAKVLERVQRIRLGDPQDENTNFGPLVSFPHMESVLGYIESGKAQKARLLCGGERVTDGAFGKGAYVAPTVFTDCRDDMTIVREEIFGPVMSILVYDDEDEAIRRANDTEYGLAAGVVTQDLARAHRAIHRLEAGICWINTWGESPAEMPVGGYKQSGVGRENGLTTLAHYTRIKSVQVELGDYASVF

General information: TITO was launched using: RESULT: Template: 2WME.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2938 -215941 -73.50 -441.60 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.95 3D Compatibility (PKB) : -73.50 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_2WME.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WME-query.scw PDB file : Tito_Scwrl_2WME.pdb: