Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSILVIADHNNQTLNGATLNVVAAAQKIGGDITVLVAGSGAQAVADAAAKVAGVSKVLLADNAAYANQLAENVAGLVADLAKGYKY--VLAASTTTGKNILPRVAALLDVSMITDIISVESANTFKRPIYAGNAIATVQSDEAIIVGTVRGTAFDPVAAEGGSAAVETVGEVKDAGVSKFVSEEIVKLDRPELTAARIVVSGGRGVGSGENYHKVLDPLADKLGAAQGASRAAVDAGFVPNDFQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKVIVAINKDEEAPINSVADYWLVGDLNAVVPELVSKL 1T9G Chain:R ((21-203)) -STLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQK--------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1T9G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain R - contact count / total energy / energy per contact / energy per residue : 999 -88345 -88.43 -488.09 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain R : 0.71 3D Compatibility (PKB) : -88.43 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_1T9G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T9G-query.scw PDB file : Tito_Scwrl_1T9G.pdb: