Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFPHHTEHHAIQRSGWLRASVLGANDGIISVTSLIMGMAASGASSHTLFITCVAGLISGATSMAAGEYISVKSQEDIEKSDLAIEAKELKKYPQKELDELTQIYISRGLSKELAKEVAIQLTTHDALGAHAR-----DEIGIHE-------NTAANPIQAALSSAASFSFGAFFPMLAILFSPEHLI-MPSVLITGIA-------ALAILGALSSYFAGTSKIKGSLRITLWGILAMAFSSWIGSLFNVTPL 5G4I Chain:A ((156-274)) --------------------------------------------------------------------------------------------------------------------EITTGLTVHEPLGAHVRALRIPDVSGIAEVDVPVLLEQSLADVDAAIASLQAAGHGVSVFLFDPLFSTEGLLQLPSGYIEGVATRVRAAGGLVISDEVQSGFGRTGS-------GMWGY----------QMFNVEP-

General information: TITO was launched using: RESULT: Template: 5G4I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 301 -19116 -63.51 -193.09 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -63.51 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.125

(partial model without unconserved sides chains): PDB file : Tito_5G4I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G4I-query.scw PDB file : Tito_Scwrl_5G4I.pdb: