Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNILDEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAAS-QSWKKIYQPLKIPRTIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYTDLRLEGKQCLSLHIPS-DADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPNLTKWLKKESKICLFAADYRLLSVDEQ--DDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQLLAGEHIGSGLGILTS
4MFZ Chain:A ((132-328))---------------------------------------------------------------------------------VYVFLATLPALRRFHATRDIPEATTWETLTQLGESVAIHRRKYGEGGTNMPWWLTLLVRGLVYRLGRLQYNLA--------VAKDGTPVLGLHIPEVGGPLIPDIYYDSLRRARPFFERHFPEHGARAATGTSWLLDPQLAEYLAEDSHILQLRRGWTLLDSEPQDGDDAILEFVFRYNGQPLEELPQRSTLEKAVVTHLLAGRH----------


General information:
TITO was launched using:
RESULT:

Template: 4MFZ.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 698 -15344 -21.98 -79.50
target 2D structure prediction score : 0.60
Monomeric hydrophicity matching model chain A : 0.67

3D Compatibility (PKB) : -21.98
2D Compatibility (Sec. Struct. Predict.) : 0.60
1D Compatibility (Hydrophobicity) : 0.67
QMean score : 0.509

(partial model without unconserved sides chains):
PDB file : Tito_4MFZ.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4MFZ-query.scw
PDB file : Tito_Scwrl_4MFZ.pdb: