Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEEQELQRGLKNRHVQL-----ISIGGAIGTGLFLGSGKSIQLAGPSILLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSIAMADLTAIGMYVRYWAPGVPQ-------WLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAIIAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELVGLVAGETENPEKVLPEAINNIPIRIILF-YLGSLFVIMAIYPWNSLNPDNSPFVEVF--SEIGITIAAS---------LINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNALTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAHSFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK 3GI9 Chain:C ((3-373)) ----------LKNKKLSLWEAVSMAVGVMIGASIFSIFGVGAKIAGRNLPETFILSGIYALLVAYSYTKLGAKIVSNAGPIAFIHKAIGDN--IITGALSILLWMSYVISIALFAKGFAGYFLPLI-APINTFNIAITEIGIVAFFTALNFFGSKAVGRAEFFIVLVKLLILGLFIFAGLITIHPSYV-------IPDLA------PSAVSGMIFASAIFFLSYMGFGVITNASEHIENPKKNVPRAIF-ISILIVMFVYVG--VAISAI--------GNLPIDELIKASENALAVAAKPFLGNLGFLLISIGALFSISSAMNATIYGGANVAYSLAKDGELPEFFERKVWFKSTEGLYITSALGVLFALLFN-------MEGVASITSAVFMVIYLFVILSHYILIDEVGGRK-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GI9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1853 -151177 -81.58 -436.93 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.70 3D Compatibility (PKB) : -81.58 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_3GI9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GI9-query.scw PDB file : Tito_Scwrl_3GI9.pdb: