Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLDKNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVIRLIRSLLERMP----DMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLLQLSDLEIGLVYLVTG--TKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTNLASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQ-LPFTHSVFGWGIALIISLVLSIWMLIALWRRIR 4EV6 Chain:A ((45-335)) ------FEDYRLIWIDCYDPKDEELYKLSKKIGISVSDLQIGLDEQEIPRVEED--EDFYLIIYKAPL---FEEDITTTSLGIYIKNNLLLTIHSDKIKAIGRLHKLISTKKPRIVFERGIGFLLYHILNEITRSYSRILMNLEDELEELEDKLLAGYDREVMEKILGLRKTLVYFHKSLIANRDVLVLLKRKYLPITTK---EDRENFEDLYYDTLQLIDMSATYREVLTSMMDITLSLENIKMNQIMKILTMVTTIFAVPMWITGIYGMNFSYLPLANNPQGFWLVMALMVVI-IMIFVYIFRR--

General information: TITO was launched using: RESULT: Template: 4EV6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1040 -92547 -88.99 -325.87 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -88.99 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_4EV6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EV6-query.scw PDB file : Tito_Scwrl_4EV6.pdb: