Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKKKVGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN 2L97 Chain:A ((98-124)) LYNHSIGDTIKITYYRNGKEETTSIKL---------------

General information: TITO was launched using: RESULT: Template: 2L97.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 42 -2516 -59.90 -93.19 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -59.90 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.879

(partial model without unconserved sides chains): PDB file : Tito_2L97.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L97-query.scw PDB file : Tito_Scwrl_2L97.pdb: