Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNFNNCVVLIIITELTLF----TNIYYIHEAQSSLIEDWAIFMAKEGKEMFQNKG 2TSC Chain:A ((50-84)) ------CHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWA---------------

General information: TITO was launched using: RESULT: Template: 2TSC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 66 -6222 -94.27 -200.71 target 2D structure prediction score : 0.00 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -94.27 2D Compatibility (Sec. Struct. Predict.) : 0.00 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.142

(partial model without unconserved sides chains): PDB file : Tito_2TSC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2TSC-query.scw PDB file : Tito_Scwrl_2TSC.pdb: