Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKFLDMSLVQRIVVGILVGILFGIFFPTW-------TFISILGTLFVSCLKAIAPILVF---FLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSEKA--PQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAF-----------RARSESTKELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEY------------LQLLLVLIGTMLFVALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLLIPLACSLFGI---SNDVAMQ---IVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGEKIKLKDCLDGLSER 3V8F Chain:A ((7-411)) ----YIEYPVLQKILIGLILGAIVGLILGHYGYAHAVHTYVKPFGDLFVRLLKMLVMPIVFASLVVGAASISPARLG-RVGVK-IVVYYLLTSAFAVTLGIIMARLFNPGAGIHLAVGGQQFQPHQAPPLVHILLDIVPTNPFGALANGQVLPTIFFAIILGIAITYLMNSENEKVRKSAETLLDAINGLAEAMYKIVNGVMQYAPIGVFALIAYCMAEQGVHVVGELAKVTAAVYVGLTLQILLVY-----FVLLKIYGIDPISFIKHA---------KDAMLTAFVTRSSSGTLPVTMRVAKEMGISEGIYSFTLPLGATINMDGTALYQGVATFFIANALGSHLTVGQQLTIVLTAVLASIGTAGVPGAGAIMLAMVLHSVGLPLTDPNVAAAYACILGIDAIL----DMGRTMVNVTGDLTGTAI-------------------------

General information: TITO was launched using: RESULT: Template: 3V8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1542 -194206 -125.94 -533.53 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -125.94 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.360

(partial model without unconserved sides chains): PDB file : Tito_3V8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V8F-query.scw PDB file : Tito_Scwrl_3V8F.pdb: