Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEYAPKAVENFMTHAKDGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRG-ALSMARSQDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAIANIETDS-SDKPKEEVKIDKINILQEAKQK 2A2N Chain:A ((28-175)) ---------------------------------------------------------------------------TSMGDIHTKLFPVECPKTVENFCVHSRNGYYNGHTFHRIIKGFMIQTGDPTGTGMGGESIWGGEFEDEFHSTLRHDRPYTLSMANAGS-NTNGSQFFI------------------TVVPT------PWLDNKHTVFGRVTKGMEVVQRISNVKVNPKTDKPYEDVSIINITV-------

General information: TITO was launched using: RESULT: Template: 2A2N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 732 -6389 -8.73 -43.76 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -8.73 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_2A2N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A2N-query.scw PDB file : Tito_Scwrl_2A2N.pdb: