Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQEQIEKKKEEKQKAEKAALEKK 3QE6 Chain:A ((159-203)) -------------------TARKDEKTAQTRESHA-------QEQLRKLQERVGRCTKEAEKMK

General information: TITO was launched using: RESULT: Template: 3QE6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 61 13340 218.68 351.04 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 218.68 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_3QE6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QE6-query.scw PDB file : Tito_Scwrl_3QE6.pdb: